Molecular genetic evidence supporting the neoplastic nature of stromal cells in 'fibrosis' after chemotherapy for testicular germ cell tumours

L. Cheng, S. Zhang, M. Wang, D. D. Davidson, M. J. Morton, J. Huang, S. Zheng, T. D. Jones, S. D. Beck, R. S. Foster

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

A residual retroperitoneal mass containing only fibrosis and necrosis is present in 40-52% of patients with advanced testicular germ cell tumours after chemotherapy. The biological nature and genetic characteristics of the stromal cells in these residual masses have not been adequately investigated. Laser-microdissected stromal cells from 27 patients who underwent retroperitoneal lymph node dissection after chemotherapy for metastatic testicular germ cell tumour were analysed. Allelic loss in the stromal cells of fibrosis was present at one or more of the ten microsatellite DNA loci examined in 23 (85%) of the cases. Chromosome arm 12p anomalies, the hallmark of germ cell neoplasia, were present in nine (33%) cases. The high frequency of allelic losses and chromosome arm 12p anomalies in the stromal cells from residual retroperitoneal fibrous masses after chemotherapy for testicular germ cell tumours suggests that the stromal cells are derived from the same tumour progenitor cells as the pre-existing metastatic germ cell tumour.

Original languageEnglish (US)
Pages (from-to)65-71
Number of pages7
JournalJournal of Pathology
Volume213
Issue number1
DOIs
StatePublished - Sep 1 2007

Keywords

  • Germ cell tumours
  • Isochromosome 12p
  • Laser capture microdissection
  • Loss of heterozygosity
  • Stroma
  • Teratoma, fluorescence in situ hybridization
  • Testicular neoplasm
  • Testis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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