Molecular genetic evidence supporting the neoplastic nature of stromal cells in 'fibrosis' after chemotherapy for testicular germ cell tumours

Liang Cheng, S. Zhang, Mingsheng Wang, Darrell Davidson, M. J. Morton, J. Huang, S. Zheng, T. D. Jones, S. D. Beck, Richard Foster

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

A residual retroperitoneal mass containing only fibrosis and necrosis is present in 40-52% of patients with advanced testicular germ cell tumours after chemotherapy. The biological nature and genetic characteristics of the stromal cells in these residual masses have not been adequately investigated. Laser-microdissected stromal cells from 27 patients who underwent retroperitoneal lymph node dissection after chemotherapy for metastatic testicular germ cell tumour were analysed. Allelic loss in the stromal cells of fibrosis was present at one or more of the ten microsatellite DNA loci examined in 23 (85%) of the cases. Chromosome arm 12p anomalies, the hallmark of germ cell neoplasia, were present in nine (33%) cases. The high frequency of allelic losses and chromosome arm 12p anomalies in the stromal cells from residual retroperitoneal fibrous masses after chemotherapy for testicular germ cell tumours suggests that the stromal cells are derived from the same tumour progenitor cells as the pre-existing metastatic germ cell tumour.

Original languageEnglish
Pages (from-to)65-71
Number of pages7
JournalJournal of Pathology
Volume213
Issue number1
DOIs
StatePublished - Sep 2007

Fingerprint

Stromal Cells
Molecular Biology
Fibrosis
Drug Therapy
Loss of Heterozygosity
Chromosomes
Germ Cell and Embryonal Neoplasms
Lymph Node Excision
Germ Cells
Microsatellite Repeats
Neoplasms
Lasers
Necrosis
Stem Cells
Testicular Germ Cell Tumor
DNA

Keywords

  • Germ cell tumours
  • Isochromosome 12p
  • Laser capture microdissection
  • Loss of heterozygosity
  • Stroma
  • Teratoma, fluorescence in situ hybridization
  • Testicular neoplasm
  • Testis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Molecular genetic evidence supporting the neoplastic nature of stromal cells in 'fibrosis' after chemotherapy for testicular germ cell tumours. / Cheng, Liang; Zhang, S.; Wang, Mingsheng; Davidson, Darrell; Morton, M. J.; Huang, J.; Zheng, S.; Jones, T. D.; Beck, S. D.; Foster, Richard.

In: Journal of Pathology, Vol. 213, No. 1, 09.2007, p. 65-71.

Research output: Contribution to journalArticle

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