Molecular imaging of bone marrow mononuclear cell homing and engraftment in ischemic myocardium

Ahmad Y. Sheikh, Shu An Lin, Feng Cao, Yuan Cao, Koen E.A. Van Der Bogt, Pauline Chu, Ching Pin Chang, Christopher H. Contag, Robert C. Robbins, Joseph C. Wu

Research output: Contribution to journalArticle

113 Scopus citations

Abstract

Bone marrow mononuclear cell (BMMC) therapy shows promise as a treatment for ischemic heart disease. However, the ability to monitor long-term cell fate remains limited. We hypothesized that molecular imaging could be used to track stem cell homing and survival after myocardial ischemia-reperfusion (I/R) injury. We first harvested donor BMMCs from adult male L2G85 transgenic mice constitutively expressing both firefly luciferase (Fluc) and enhanced green fluorescence protein reporter gene. Fluorescence-activated cell sorting analysis revealed ∼0.07% of the population to consist of classic hematopoietic stem cells (lin-, thy-int, c-kit+, Sca-1+). Afterward, adult female FVB recipients (n = 38) were randomized to sham surgery or acute I/R injury. Animals in the sham (n = 16) and I/R (n = 22) groups received 5 × 106 of the L2G85-derived BMMCs via tail vein injection. Bioluminescence imaging (BLI) was used to track cell migration and survival in vivo for 4 weeks. BLI showed preferential homing of BMMCs to hearts with I/R injury compared with sham hearts within the first week following cell injection. Ex vivo analysis of explanted hearts by histology confirmed BLI imaging results, and quantitative real-time polymerase chain reaction (for the male Sry gene) further demonstrated a greater number of BMMCs in hearts with I/R injury compared with the sham group. Functional evaluation by echocardiography demonstrated a trend toward improved left ventricular fractional shortening in animals receiving BMMCs. Taken together, these data demonstrate that molecular imaging can be used to successfully track BMMC therapy in murine models of heart disease. Specifically, we have demonstrated that systemically delivered BMMCs preferentially home to and are retained by injured myocardium.

Original languageEnglish (US)
Pages (from-to)2677-2684
Number of pages8
JournalSTEM CELLS
Volume25
Issue number10
DOIs
StatePublished - Oct 2007

Keywords

  • Bone marrow
  • Cell homing
  • Heart diseases
  • Molecular imaging

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

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    Sheikh, A. Y., Lin, S. A., Cao, F., Cao, Y., Van Der Bogt, K. E. A., Chu, P., Chang, C. P., Contag, C. H., Robbins, R. C., & Wu, J. C. (2007). Molecular imaging of bone marrow mononuclear cell homing and engraftment in ischemic myocardium. STEM CELLS, 25(10), 2677-2684. https://doi.org/10.1634/stemcells.2007-0041