Molecular karyotype alterations induced by transformation in Aspergillus nidulans are mitotically stable

Xiaoling Xuei, Paul L. Skatrud

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Clamped homogeneous electric field (CHEF)-gel electrophoresis was used to define the electrophoretic molecular karyotype of Aspergillus nidulans strain OC-1 before and after protoplast-based genetic transformation. The transforming DNA caused alterations in the molecular karyotypes in all transformants examined. Rather dramatic changes were observed in karyotypes, including apparent chromosome loss, massive size alterations, and the appearance of large chromosomes. Changes in molecular karyotype were mitotically stable.

Original languageEnglish (US)
Pages (from-to)225-227
Number of pages3
JournalCurrent Genetics
Volume26
Issue number3
DOIs
StatePublished - Sep 1 1994
Externally publishedYes

Keywords

  • Aspergillus nidulans
  • Genetic transformation
  • Mitotic stability
  • Molecular karyotype

ASJC Scopus subject areas

  • Genetics

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