Molecular tools for analysis of gene function in parasitic microorganisms

Markus Meissner, Carolina Agop-Nersesian, William Sullivan

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

With the completion of several genome sequences for parasitic protozoa, research in molecular parasitology entered the "post-genomic" era. Accompanied by global transcriptome and proteome analysis, huge datasets have been generated that have added many novel candidates to the list of drug and vaccine targets. The challenge is now to validate these factors and to bring science back to the bench to perform a detailed characterization. In some parasites, like Trypanosoma brucei, high-throughput genetic screens have been established using RNA interference [for a detailed review, see Motyka and Englund (2004)]. In most protozoan parasites, however, more time-consuming approaches have to be employed to identify and characterize the function of promising candidates in detail. This review aims to summarize the status of molecular genetic tools available for a variety of protozoan pathogens and discuss how they can be implemented to advance our understanding of parasite biology.

Original languageEnglish
Pages (from-to)963-975
Number of pages13
JournalApplied Microbiology and Biotechnology
Volume75
Issue number5
DOIs
StatePublished - Jul 2007

Fingerprint

Microorganisms
Parasites
Genes
Protozoa
Parasitology
Trypanosoma brucei brucei
Vaccines
Gene Expression Profiling
Pathogens
Proteome
RNA Interference
RNA
Molecular Biology
Throughput
Genome
Proteins
Research
Pharmaceutical Preparations
Datasets

Keywords

  • Inducible system
  • Protozoan parasites
  • Recombinase
  • RNA interference
  • Transcription
  • Transfection

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Bioengineering
  • Microbiology (medical)

Cite this

Molecular tools for analysis of gene function in parasitic microorganisms. / Meissner, Markus; Agop-Nersesian, Carolina; Sullivan, William.

In: Applied Microbiology and Biotechnology, Vol. 75, No. 5, 07.2007, p. 963-975.

Research output: Contribution to journalArticle

Meissner, Markus ; Agop-Nersesian, Carolina ; Sullivan, William. / Molecular tools for analysis of gene function in parasitic microorganisms. In: Applied Microbiology and Biotechnology. 2007 ; Vol. 75, No. 5. pp. 963-975.
@article{4b7cfd4ca3704e54bfa12f6e8d886e9e,
title = "Molecular tools for analysis of gene function in parasitic microorganisms",
abstract = "With the completion of several genome sequences for parasitic protozoa, research in molecular parasitology entered the {"}post-genomic{"} era. Accompanied by global transcriptome and proteome analysis, huge datasets have been generated that have added many novel candidates to the list of drug and vaccine targets. The challenge is now to validate these factors and to bring science back to the bench to perform a detailed characterization. In some parasites, like Trypanosoma brucei, high-throughput genetic screens have been established using RNA interference [for a detailed review, see Motyka and Englund (2004)]. In most protozoan parasites, however, more time-consuming approaches have to be employed to identify and characterize the function of promising candidates in detail. This review aims to summarize the status of molecular genetic tools available for a variety of protozoan pathogens and discuss how they can be implemented to advance our understanding of parasite biology.",
keywords = "Inducible system, Protozoan parasites, Recombinase, RNA interference, Transcription, Transfection",
author = "Markus Meissner and Carolina Agop-Nersesian and William Sullivan",
year = "2007",
month = "7",
doi = "10.1007/s00253-007-0946-4",
language = "English",
volume = "75",
pages = "963--975",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "5",

}

TY - JOUR

T1 - Molecular tools for analysis of gene function in parasitic microorganisms

AU - Meissner, Markus

AU - Agop-Nersesian, Carolina

AU - Sullivan, William

PY - 2007/7

Y1 - 2007/7

N2 - With the completion of several genome sequences for parasitic protozoa, research in molecular parasitology entered the "post-genomic" era. Accompanied by global transcriptome and proteome analysis, huge datasets have been generated that have added many novel candidates to the list of drug and vaccine targets. The challenge is now to validate these factors and to bring science back to the bench to perform a detailed characterization. In some parasites, like Trypanosoma brucei, high-throughput genetic screens have been established using RNA interference [for a detailed review, see Motyka and Englund (2004)]. In most protozoan parasites, however, more time-consuming approaches have to be employed to identify and characterize the function of promising candidates in detail. This review aims to summarize the status of molecular genetic tools available for a variety of protozoan pathogens and discuss how they can be implemented to advance our understanding of parasite biology.

AB - With the completion of several genome sequences for parasitic protozoa, research in molecular parasitology entered the "post-genomic" era. Accompanied by global transcriptome and proteome analysis, huge datasets have been generated that have added many novel candidates to the list of drug and vaccine targets. The challenge is now to validate these factors and to bring science back to the bench to perform a detailed characterization. In some parasites, like Trypanosoma brucei, high-throughput genetic screens have been established using RNA interference [for a detailed review, see Motyka and Englund (2004)]. In most protozoan parasites, however, more time-consuming approaches have to be employed to identify and characterize the function of promising candidates in detail. This review aims to summarize the status of molecular genetic tools available for a variety of protozoan pathogens and discuss how they can be implemented to advance our understanding of parasite biology.

KW - Inducible system

KW - Protozoan parasites

KW - Recombinase

KW - RNA interference

KW - Transcription

KW - Transfection

UR - http://www.scopus.com/inward/record.url?scp=34250657018&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250657018&partnerID=8YFLogxK

U2 - 10.1007/s00253-007-0946-4

DO - 10.1007/s00253-007-0946-4

M3 - Article

VL - 75

SP - 963

EP - 975

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 5

ER -