Monitoring of cell therapy and assessment of cardiac function using magnetic resonance imaging in a mouse model of myocardial infarction

Linda W. Van Laake, Robert Passier, Jantine Monshouwer-Kloots, Marcel G. Nederhoff, Dorien Ward-Van Oostwaard, Loren J. Field, Cees J. Van Echteld, Pieter A. Doevendans, Christine L. Mummery

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

We have developed a mouse severe combined immunodeficient (SCID) model of myocardial infarction based on permanent coronary artery occlusion that allows long-term functional analysis of engrafted human embryonic stem cell-derived cardiomyocytes, genetically marked with green fluorescent protein (GFP), in the mouse heart. We describe methods for delivery of dissociated cardiomyocytes to the left ventricle that minimize scar formation and visualization and validation of the identity of the engrafted cells using the GFP emission spectrum, and histological techniques compatible with GFP epifluorescence, for monitoring phenotypic changes in the grafts in vivo. In addition, we describe how magnetic resonance imaging can be adapted for use in mice to monitor cardiac function non-invasively and repeatedly. The model can be adapted to include multiple control or other cell populations. The procedure for a cohort of six mice can be completed in a maximum of 13 weeks, depending on follow-up, with 30 h of hands-on time.

Original languageEnglish (US)
Pages (from-to)2551-2567
Number of pages17
JournalNature protocols
Volume2
Issue number10
DOIs
StatePublished - Oct 2007

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Van Laake, L. W., Passier, R., Monshouwer-Kloots, J., Nederhoff, M. G., Ward-Van Oostwaard, D., Field, L. J., Van Echteld, C. J., Doevendans, P. A., & Mummery, C. L. (2007). Monitoring of cell therapy and assessment of cardiac function using magnetic resonance imaging in a mouse model of myocardial infarction. Nature protocols, 2(10), 2551-2567. https://doi.org/10.1038/nprot.2007.371