Monitoring temperature and light exposure of biosamples exposed to ultraviolet and low energy radiation

Hsin Kai Chen, Gabi Nindl Waite, Penney L. Miller, Ellen F. Hughes, Lee R. Waite

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We previously showed that T-lymphocytes produce catalytic amounts of hydrogen peroxide (H2O2) in a membrane-associated process when irradiated with narrowband ultraviolet B (UVB) light. This form of phototherapy is thought to be highly effective for treatment of inflammatory skin diseases such as psoriasis, but also includes the potential for severe burning and development of skin cancer. Consequently, information on the therapeutic mechanism of narrowband UVB phototherapy and its regulation is warranted. Our laboratory is researching the mechanistic involvement of T-cell H2O2 production and its potential regulation by low energy electromagnetic field (EMF) radiation, which has been shown to beneficially influence inflammatory diseases such as psoriasis. To study photochemical H 2O2 production in small samples such as suspensions of T-lymphocyte cell extracts, we use a reactor in which 12 microliter-sized samples are exposed to UVB. We simultaneously operate two identical systems, one for experimental, the other for control samples, within a walk-in environmental chamber maintained at 37 °C. The current paper addresses the control of UVB light exposure and temperature in our experimental setup. We quantified UVB light by radiometric spot measurements and by chemical potassium ferrioxalate actinometry. We modified the actinometer so that UVB light of 5-hour experiments could be detected. Temperature was controlled by air convection and remained constant within 0.5 °C in air and liquid samples. Preliminary data of the effect of low energy EMF radiation on T-cell H2O2 production are presented.

Original languageEnglish
Title of host publicationTechnical Papers of ISA
Pages312-317
Number of pages6
Volume468
StatePublished - 2007
Event44th Annual Rocky Mountain Bioengineering Symposium and 44th International ISA Biomedical Sciences Instrumentation Symposium - Denver, CO, United States
Duration: Apr 13 2007Apr 15 2007

Other

Other44th Annual Rocky Mountain Bioengineering Symposium and 44th International ISA Biomedical Sciences Instrumentation Symposium
CountryUnited States
CityDenver, CO
Period4/13/074/15/07

Fingerprint

T-cells
Radiation
Monitoring
Electromagnetic fields
Skin
Environmental chambers
Temperature
Air
Hydrogen peroxide
Potassium
Membranes
Liquids
Experiments

Keywords

  • EMF
  • Hydrogen peroxide
  • Potassium ferrioxalate actinometer
  • T-lymphocytes
  • UVB light exposure

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering
  • Computer Science Applications

Cite this

Chen, H. K., Waite, G. N., Miller, P. L., Hughes, E. F., & Waite, L. R. (2007). Monitoring temperature and light exposure of biosamples exposed to ultraviolet and low energy radiation. In Technical Papers of ISA (Vol. 468, pp. 312-317)

Monitoring temperature and light exposure of biosamples exposed to ultraviolet and low energy radiation. / Chen, Hsin Kai; Waite, Gabi Nindl; Miller, Penney L.; Hughes, Ellen F.; Waite, Lee R.

Technical Papers of ISA. Vol. 468 2007. p. 312-317.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chen, HK, Waite, GN, Miller, PL, Hughes, EF & Waite, LR 2007, Monitoring temperature and light exposure of biosamples exposed to ultraviolet and low energy radiation. in Technical Papers of ISA. vol. 468, pp. 312-317, 44th Annual Rocky Mountain Bioengineering Symposium and 44th International ISA Biomedical Sciences Instrumentation Symposium, Denver, CO, United States, 4/13/07.
Chen HK, Waite GN, Miller PL, Hughes EF, Waite LR. Monitoring temperature and light exposure of biosamples exposed to ultraviolet and low energy radiation. In Technical Papers of ISA. Vol. 468. 2007. p. 312-317
Chen, Hsin Kai ; Waite, Gabi Nindl ; Miller, Penney L. ; Hughes, Ellen F. ; Waite, Lee R. / Monitoring temperature and light exposure of biosamples exposed to ultraviolet and low energy radiation. Technical Papers of ISA. Vol. 468 2007. pp. 312-317
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