Monoclonal antibody (II2C) to human lactoferrin inactivates the myelopoietic suppressive effect of human lactoferrin in vitro

G. W. Sledge, D. C. Bicknell, E. L. Harris, G. Zegarra, H. E. Broxmeyer

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A mouse monoclonal antibody was prepared against purified and fully iron-saturated human breast milk lactoferrin (LF). This antibody was of the IgG1 subclass, and recognized LF biosynthesized in low-density normal human bone marrow cells and LF stored in normal human polymorphonuclear neutrophils. This antibody did not recognize purified and iron-saturated human transferrin. The antibody inactivated the suppressive effects of purified and iron-saturated human milk LF and LF present in crude extracts of normal polymorphonuclear neutrophils against the release of granulocyte-macrophage colony-stimulating factors from mononuclear blood leukocytes in vitro.

Original languageEnglish (US)
Pages (from-to)333-337
Number of pages5
JournalExperimental Hematology
Volume14
Issue number5
StatePublished - Sep 24 1986

Fingerprint

Lactoferrin
Monoclonal Antibodies
Human Milk
Iron
Antibodies
Neutrophils
Mononuclear Leukocytes
Granulocyte-Macrophage Colony-Stimulating Factor
Transferrin
Complex Mixtures
Bone Marrow Cells
Immunoglobulin G
In Vitro Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

Cite this

Monoclonal antibody (II2C) to human lactoferrin inactivates the myelopoietic suppressive effect of human lactoferrin in vitro. / Sledge, G. W.; Bicknell, D. C.; Harris, E. L.; Zegarra, G.; Broxmeyer, H. E.

In: Experimental Hematology, Vol. 14, No. 5, 24.09.1986, p. 333-337.

Research output: Contribution to journalArticle

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