Monoclonal ligand binding site related anti-idiotypic antibodies elicited with a polyclonal kinin antibody

C. E. Odya, R. Yapa, B. Soltani-Tehrani, R. J. Carlin, C. H. Lee

Research output: Contribution to journalArticle

Abstract

Splenocytes from mice immunized with homogenous, polyclonal, rabbit kinin antibody (BK21) were fused using polyethylene glycol with the mouse myeloma cell line SP2/o. Eleven monoclonal antibodies, whose binding to BK21 could be inhibited by bradykinin, were obtained from 3 fusions. All of these anti- idiotypic antibodies were of the IgG(1k) isotype, except for one, which was an IgG(2ak). An IgM(k), auto-anti-idiotypic antibody, reactive with BK21 was obtained from a fusion of SP2/o cells and splenocytes from a mouse immunized with bradykinin conjugated with carbodiimide to keyhole limpet hemocyanin. Bradykinin could completely inhibit the binding of all of the anti-idiotypic antibodies to BK21 in an enzyme-linked immunosorbent assay. This result is consistent with the anti-idiotypic antibodies being reactive with the ligand binding sites of BK21. It was possible to separate the anti-idiotypic antibodies into 2 groups. The first group, 10 of the 12 antibodies tested, was more sensitive to inhibition by bradykinin than the second group and was not readily inhibited by des-Arg9-bradykinin. The second group was about 7 times more sensitive to inhibition by des-Arg9-bradykinin than by bradykinin. Further experiments will be needed to determine whether or not these monoclonal anti-idiotypic antibodies are 'internal image' antibodies.

Original languageEnglish (US)
Pages (from-to)45-53
Number of pages9
JournalHybridoma
Volume12
Issue number1
DOIs
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • Immunology
  • Genetics

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