Monocyte chemoattractant protein-1 and RANTES are chemotactic for graft infiltrating lymphocytes during acute lung allograft rejection

Y. Sekine, K. Yasufuku, K. M. Heidler, Oscar Cummings, N. Van Rooijen, T. Fujisawa, John Brown, D. S. Wilkes

Research output: Contribution to journalArticle

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Abstract

Graft infiltrating lymphocytes (GILs) are crucial to rejection of lung allografts. However, chemotactic activities, chemokines responsible for GIL recruitment, and cells involved in chemokine production during lung allograft rejection have not been evaluated. This study determined whether chemotactic activity for GILs is upregulated, and whether the chemokines monocyte chemoattractant protein (MCP)-1 and regulated on activation, normal T cells expressed and secreted (RANTES) have roles in GIL chemotaxis during lung allograft rejection. F344 (RT1Iv1) rat lung allografts were transplanted into WKY (RT1I) recipients. Chemotactic activity for GILs and quantities of MCP-1 and RANTES were determined in allograft bronchoalveolar lavage fluid 1 wk after transplantation. Data showed that during rejection, chemotactic activity for GILs is upregulated, MCP-1 and RANTES are produced locally, and both MCP-1 and RANTES are operative in GIL recruitment. Immunohistochemistry showed that alveolar macrophages (AMs) were the major source of MCP-1 and that other lung cells, including AMs, were the source of RANTES. Further, depletion of AMs in the donor lung before transplantation downregulated chemotaxis for GILs and production of MCP-1 during rejection episodes. These data show that chemotaxis for GILs is upregulated locally during lung allograft rejection, and that MCP-1 and RANTES contribute to GIL recruitment during the rejection response.

Original languageEnglish
Pages (from-to)719-726
Number of pages8
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume23
Issue number6
StatePublished - 2000

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T-cells
Lymphocytes
Chemokine CCL2
Grafts
Allografts
Chemical activation
T-Lymphocytes
Transplants
Lung
Alveolar Macrophages
Chemotaxis
Chemokines
Rejection (Psychology)
Transplantation (surgical)
Lung Transplantation
Inbred F344 Rats
Bronchoalveolar Lavage Fluid
Rats
Down-Regulation
Transplantation

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Monocyte chemoattractant protein-1 and RANTES are chemotactic for graft infiltrating lymphocytes during acute lung allograft rejection. / Sekine, Y.; Yasufuku, K.; Heidler, K. M.; Cummings, Oscar; Van Rooijen, N.; Fujisawa, T.; Brown, John; Wilkes, D. S.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 23, No. 6, 2000, p. 719-726.

Research output: Contribution to journalArticle

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abstract = "Graft infiltrating lymphocytes (GILs) are crucial to rejection of lung allografts. However, chemotactic activities, chemokines responsible for GIL recruitment, and cells involved in chemokine production during lung allograft rejection have not been evaluated. This study determined whether chemotactic activity for GILs is upregulated, and whether the chemokines monocyte chemoattractant protein (MCP)-1 and regulated on activation, normal T cells expressed and secreted (RANTES) have roles in GIL chemotaxis during lung allograft rejection. F344 (RT1Iv1) rat lung allografts were transplanted into WKY (RT1I) recipients. Chemotactic activity for GILs and quantities of MCP-1 and RANTES were determined in allograft bronchoalveolar lavage fluid 1 wk after transplantation. Data showed that during rejection, chemotactic activity for GILs is upregulated, MCP-1 and RANTES are produced locally, and both MCP-1 and RANTES are operative in GIL recruitment. Immunohistochemistry showed that alveolar macrophages (AMs) were the major source of MCP-1 and that other lung cells, including AMs, were the source of RANTES. Further, depletion of AMs in the donor lung before transplantation downregulated chemotaxis for GILs and production of MCP-1 during rejection episodes. These data show that chemotaxis for GILs is upregulated locally during lung allograft rejection, and that MCP-1 and RANTES contribute to GIL recruitment during the rejection response.",
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AU - Heidler, K. M.

AU - Cummings, Oscar

AU - Van Rooijen, N.

AU - Fujisawa, T.

AU - Brown, John

AU - Wilkes, D. S.

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