Monomeric IgG2a promotes maturation of bone-marrow macrophages and expression of the mannose receptor

Stefan Schreiber, Janice Blum, William F. Stenson, Richard P. Macdermott, Philip D. Stahl, Steven L. Teitelbaum, Sherrie L. Perkins

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The macrophage mannose receptor, a 172-kDa lineage-specific glycoprotein, partakes in nonopsonin-mediated phagocytosis by recognition of terminal mannose residues on targeted particles. Because appearance of the receptor progresses with monocyte/macrophage differentiation, its expression is indicative of the maturational state of the cell. Monomeric IgG2a and IgG2b up-regulate mannose-receptor surface expression and biosynthesis by murine bone-marrow macrophage precursors as much as 7- to 12-fold in a dose-dependent manner. IgG2a accelerates macrophage mannose-receptor expression by several days during in vitro bone-marrow differentiation; however, treated and control cells ultimately express equivalent levels of receptor. Moreover, the effect is independent of cell cycle or ambient levels of colony-stimulating factor 1. The coinduction of another maturation-dependent lineage-specific antigen, F4/80, and the fact that macrophage precursors respond to IgG2a only within the first day of culture, indicate that the targeted cell is an early myelomonocytic precursor, responsive only during a short, early developmental window. The effect is specific for immunoglobulin molecules of the IgG2a and IgG2b subclasses and probably involves an Fcγ-receptor signal-transduction pathway but not macrophage priming or activation. Most importantly, a paracrine mechanism of immunoglobulin-mediated bone-marrow macrophage differentiation is suggested by experiments in which basal levels of mannose-receptor expression are reduced by continual removal of B-cell-generated IgG from marrow cultures. Thus, IgG2a and IgG2b prompt mannose-receptor synthesis and bone-marrow macrophage differentiation and may, therefore, play a role in the regulation of macrophage differentiation in host defense.

Original languageEnglish (US)
Pages (from-to)1616-1620
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number5
StatePublished - 1991
Externally publishedYes

Fingerprint

Bone Marrow
Macrophages
Immunoglobulins
mannose receptor
Macrophage Colony-Stimulating Factor
Fc Receptors
Mannose
Phagocytosis
Monocytes
Signal Transduction
Glycoproteins
Cell Cycle
B-Lymphocytes
Up-Regulation
Immunoglobulin G

Keywords

  • Bone-marrow macrophage differentiation
  • Immunoglobulins
  • Macrophage mannose receptor

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Schreiber, S., Blum, J., Stenson, W. F., Macdermott, R. P., Stahl, P. D., Teitelbaum, S. L., & Perkins, S. L. (1991). Monomeric IgG2a promotes maturation of bone-marrow macrophages and expression of the mannose receptor. Proceedings of the National Academy of Sciences of the United States of America, 88(5), 1616-1620.

Monomeric IgG2a promotes maturation of bone-marrow macrophages and expression of the mannose receptor. / Schreiber, Stefan; Blum, Janice; Stenson, William F.; Macdermott, Richard P.; Stahl, Philip D.; Teitelbaum, Steven L.; Perkins, Sherrie L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 5, 1991, p. 1616-1620.

Research output: Contribution to journalArticle

Schreiber, Stefan ; Blum, Janice ; Stenson, William F. ; Macdermott, Richard P. ; Stahl, Philip D. ; Teitelbaum, Steven L. ; Perkins, Sherrie L. / Monomeric IgG2a promotes maturation of bone-marrow macrophages and expression of the mannose receptor. In: Proceedings of the National Academy of Sciences of the United States of America. 1991 ; Vol. 88, No. 5. pp. 1616-1620.
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