Morphological and functional characteristics of three-dimensional engineered bone-ligament-bone constructs following implantation

Jinjin Ma, Kristen Goble, Michael Smietana, Tatiana Kostrominova, Lisa Larkin, Ellen M. Arruda

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The incidence of ligament injury has recently been estimated at 400,000/year. The preferred treatment is reconstruction using an allograft, but outcomes are limited by donor availability, biomechanical incompatibility, and immune rejection. The creation of an engineered ligament in vitro solely from patient bone marrow stromal cells (has the potential to greatly enhance outcomes in knee reconstructions. Our laboratory has developed a scaffoldless method to engineer three-dimensional (3D) ligament and bone constructs from rat bone marrow stem cells in vitro. Coculture of these two engineered constructs results in a 3D bone-ligament-bone (BLB) construct with viable entheses, which was successfully used for medial collateral ligament (MCL) replacement in a rat model. 1 month and 2 month implantations were applied to the engineered BLBs. Implantation of 3D BLBs in a MCL replacement application demonstrated that our in vitro engineered tissues grew and remodeled quickly in vivo to an advanced phenotype and partially restored function of the knee. The explanted 3D BLB ligament region stained positively for type I collagen and elastin and was well vascularized after 1 and 2 months in vivo. Tangent moduli of the ligament portion of the 3D BLB 1 month explants increased by a factor of 2.4 over in vitro controls, to a value equivalent to those observed in 14-day-old neonatal rat MCLs. The 3D BLB 1 month explants also exhibited a functionally graded response that closely matched native MCL inhomogeneity, indicating the constructs functionally adapted in vivo.

Original languageEnglish
Article number101017-1
JournalJournal of Biomechanical Engineering
Volume131
Issue number10
DOIs
StatePublished - Oct 2009

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Ligaments
Bone
Bone and Bones
Collateral Ligaments
Rats
Knee
Elastin
Coculture Techniques
Collagen Type I
Mesenchymal Stromal Cells
Bone Marrow Cells
Allografts
Stem Cells
Stem cells
Collagen
Tissue Donors
Phenotype
In Vitro Techniques
Incidence
Wounds and Injuries

ASJC Scopus subject areas

  • Biomedical Engineering
  • Physiology (medical)

Cite this

Morphological and functional characteristics of three-dimensional engineered bone-ligament-bone constructs following implantation. / Ma, Jinjin; Goble, Kristen; Smietana, Michael; Kostrominova, Tatiana; Larkin, Lisa; Arruda, Ellen M.

In: Journal of Biomechanical Engineering, Vol. 131, No. 10, 101017-1, 10.2009.

Research output: Contribution to journalArticle

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