Morphological and membrane characteristics of spider and spindle cells isolated from rabbit sinus node

Jianyi Wu, Richard B. Schuessler, Mark D. Rodefeld, Jeffrey E. Saffitz, John P. Boineau

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

This study reports the comparative quantitative, morphological, and electrophysiological properties of two pacemaker cell types, spider and spindle-shaped cells, isolated from the rabbit sinoatrial node. Isolated nodal cells were studied with perforated and ruptured patch whole cell recording techniques. The basic spontaneous cycle length of the spider cells was 381 ± 12 ms, and the basic spontaneous cycle length of the spindle cells was 456 ± 17 ms (n = 12, P < 0.05). The spider cells had a more positive maximum diastolic potential (-54 ± 1 mV) compared with the spindle cells (-68 ± 1mV, P < 0.05). The overshoot and action potential amplitudes were also smaller in the spider cells. The hyperpolarization-activated inward (If) current density, measured from their tail currents, was 15 ± 1.3 pA/pF for the spider cells and 9 ± 0.7 pA/pF for the spindle cells (P < 0.01). If current activation voltage was more positive in the spider cells than the spindle cells. Isoproterenol (1 μM) decreased the spontaneous cycle length of the spider cells by 28 ± 3% and the spindle cells by 20 ± 1.5% (P < 0.05). Acetylcholine (0.5 μM) hyperpolarized the membrane potential of the spider cells to -86 ± 0.7mV and the spindle cells to -76 ± 0.8 mV (P < 0.05). In summary, there are at least two distinct pacemaker cell types in the sinus node with different electrophysiological characteristics.

Original languageEnglish (US)
Pages (from-to)H1232-H1240
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume280
Issue number3 49-3
StatePublished - Jun 26 2001

Fingerprint

Spiders
Sinoatrial Node
Rabbits
Membranes
Patch-Clamp Techniques

Keywords

  • Hyperpolarization-activated inward current
  • Pacemaker current

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Morphological and membrane characteristics of spider and spindle cells isolated from rabbit sinus node. / Wu, Jianyi; Schuessler, Richard B.; Rodefeld, Mark D.; Saffitz, Jeffrey E.; Boineau, John P.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 280, No. 3 49-3, 26.06.2001, p. H1232-H1240.

Research output: Contribution to journalArticle

Wu, Jianyi ; Schuessler, Richard B. ; Rodefeld, Mark D. ; Saffitz, Jeffrey E. ; Boineau, John P. / Morphological and membrane characteristics of spider and spindle cells isolated from rabbit sinus node. In: American Journal of Physiology - Heart and Circulatory Physiology. 2001 ; Vol. 280, No. 3 49-3. pp. H1232-H1240.
@article{2cf6acd97f884dd69f4b109db97b353a,
title = "Morphological and membrane characteristics of spider and spindle cells isolated from rabbit sinus node",
abstract = "This study reports the comparative quantitative, morphological, and electrophysiological properties of two pacemaker cell types, spider and spindle-shaped cells, isolated from the rabbit sinoatrial node. Isolated nodal cells were studied with perforated and ruptured patch whole cell recording techniques. The basic spontaneous cycle length of the spider cells was 381 ± 12 ms, and the basic spontaneous cycle length of the spindle cells was 456 ± 17 ms (n = 12, P < 0.05). The spider cells had a more positive maximum diastolic potential (-54 ± 1 mV) compared with the spindle cells (-68 ± 1mV, P < 0.05). The overshoot and action potential amplitudes were also smaller in the spider cells. The hyperpolarization-activated inward (If) current density, measured from their tail currents, was 15 ± 1.3 pA/pF for the spider cells and 9 ± 0.7 pA/pF for the spindle cells (P < 0.01). If current activation voltage was more positive in the spider cells than the spindle cells. Isoproterenol (1 μM) decreased the spontaneous cycle length of the spider cells by 28 ± 3{\%} and the spindle cells by 20 ± 1.5{\%} (P < 0.05). Acetylcholine (0.5 μM) hyperpolarized the membrane potential of the spider cells to -86 ± 0.7mV and the spindle cells to -76 ± 0.8 mV (P < 0.05). In summary, there are at least two distinct pacemaker cell types in the sinus node with different electrophysiological characteristics.",
keywords = "Hyperpolarization-activated inward current, Pacemaker current",
author = "Jianyi Wu and Schuessler, {Richard B.} and Rodefeld, {Mark D.} and Saffitz, {Jeffrey E.} and Boineau, {John P.}",
year = "2001",
month = "6",
day = "26",
language = "English (US)",
volume = "280",
pages = "H1232--H1240",
journal = "American Journal of Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "3 49-3",

}

TY - JOUR

T1 - Morphological and membrane characteristics of spider and spindle cells isolated from rabbit sinus node

AU - Wu, Jianyi

AU - Schuessler, Richard B.

AU - Rodefeld, Mark D.

AU - Saffitz, Jeffrey E.

AU - Boineau, John P.

PY - 2001/6/26

Y1 - 2001/6/26

N2 - This study reports the comparative quantitative, morphological, and electrophysiological properties of two pacemaker cell types, spider and spindle-shaped cells, isolated from the rabbit sinoatrial node. Isolated nodal cells were studied with perforated and ruptured patch whole cell recording techniques. The basic spontaneous cycle length of the spider cells was 381 ± 12 ms, and the basic spontaneous cycle length of the spindle cells was 456 ± 17 ms (n = 12, P < 0.05). The spider cells had a more positive maximum diastolic potential (-54 ± 1 mV) compared with the spindle cells (-68 ± 1mV, P < 0.05). The overshoot and action potential amplitudes were also smaller in the spider cells. The hyperpolarization-activated inward (If) current density, measured from their tail currents, was 15 ± 1.3 pA/pF for the spider cells and 9 ± 0.7 pA/pF for the spindle cells (P < 0.01). If current activation voltage was more positive in the spider cells than the spindle cells. Isoproterenol (1 μM) decreased the spontaneous cycle length of the spider cells by 28 ± 3% and the spindle cells by 20 ± 1.5% (P < 0.05). Acetylcholine (0.5 μM) hyperpolarized the membrane potential of the spider cells to -86 ± 0.7mV and the spindle cells to -76 ± 0.8 mV (P < 0.05). In summary, there are at least two distinct pacemaker cell types in the sinus node with different electrophysiological characteristics.

AB - This study reports the comparative quantitative, morphological, and electrophysiological properties of two pacemaker cell types, spider and spindle-shaped cells, isolated from the rabbit sinoatrial node. Isolated nodal cells were studied with perforated and ruptured patch whole cell recording techniques. The basic spontaneous cycle length of the spider cells was 381 ± 12 ms, and the basic spontaneous cycle length of the spindle cells was 456 ± 17 ms (n = 12, P < 0.05). The spider cells had a more positive maximum diastolic potential (-54 ± 1 mV) compared with the spindle cells (-68 ± 1mV, P < 0.05). The overshoot and action potential amplitudes were also smaller in the spider cells. The hyperpolarization-activated inward (If) current density, measured from their tail currents, was 15 ± 1.3 pA/pF for the spider cells and 9 ± 0.7 pA/pF for the spindle cells (P < 0.01). If current activation voltage was more positive in the spider cells than the spindle cells. Isoproterenol (1 μM) decreased the spontaneous cycle length of the spider cells by 28 ± 3% and the spindle cells by 20 ± 1.5% (P < 0.05). Acetylcholine (0.5 μM) hyperpolarized the membrane potential of the spider cells to -86 ± 0.7mV and the spindle cells to -76 ± 0.8 mV (P < 0.05). In summary, there are at least two distinct pacemaker cell types in the sinus node with different electrophysiological characteristics.

KW - Hyperpolarization-activated inward current

KW - Pacemaker current

UR - http://www.scopus.com/inward/record.url?scp=0034967372&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034967372&partnerID=8YFLogxK

M3 - Article

C2 - 11179068

AN - SCOPUS:0034967372

VL - 280

SP - H1232-H1240

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6135

IS - 3 49-3

ER -