Morphological transformation in esophageal submucosa by bone marrow cells

Esophageal implantation under external esophageal perfusion

Yan Li, John Wo, Susan Ellis, Mukunda B. Ray, Whitney Jones, Robert C G Martin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Accumulating clinical and experimental studies indicate that Barrett's esophagus might arise through multipotential stem cells under the stress of gastroesopliageal reflux. Previously, we have presented a novel external pump perfusion rat model and demonstrated that perfusion with both acid and bile can induce severe esophagitis in 1 week with a similarly pathological change seen in humans. The aim of this study was to investigate the histological changes of esophagus after bone marrow cell engraftment with bile and acid perfusion. The external pump perfusion procedure involved implantation of a microosmotic pump for esophageal perfusion. Bone marrow cells were obtained by flushing of the femur marrow, and the cell suspension was injected between the esophageal muscular and inner mucosa layer. Histological changes were determined after 4 weeks of perfusion. Proliferating cell nuclear antigen, 8-hydroxy- deoxyguanosine, manganese superoxide dismutase, and apoptosis were measured by immunohistochemical staining and TUNEL assay, respectively. Severe esophagitis was seen in both acid and bile perfusion. Bone marrow engraftment and potentiation was seen in both the acid and bile perfusion, when compared to saline controls. Glandular-like cells in submucosa, consistent with intestinal metaplasia, confirmed by Alcin Blue-PAS staining were observed after bone marrow esophageal implantation along with bile perfusion, but not with acid perfusion and controls. Bone marrow implantation in conjunction with esophageal reflux injury contributes to abnormal histological changes consistent with early Barrett's esophageal changes. Engrafted bone marrow cells proliferate under oxidative stress conditions with bile perfusion.

Original languageEnglish (US)
Pages (from-to)697-705
Number of pages9
JournalStem Cells and Development
Volume15
Issue number5
DOIs
StatePublished - Oct 2006
Externally publishedYes

Fingerprint

Bone Marrow Cells
Perfusion
Bile Acids and Salts
Infusion Pumps
Bone Marrow
Esophagitis
Bile
Staining and Labeling
Deoxyguanosine
Barrett Esophagus
In Situ Nick-End Labeling
Proliferating Cell Nuclear Antigen
Metaplasia
Gastroesophageal Reflux
Femur
Esophagus
Superoxide Dismutase
Suspensions
Mucous Membrane
Oxidative Stress

ASJC Scopus subject areas

  • Hematology

Cite this

Morphological transformation in esophageal submucosa by bone marrow cells : Esophageal implantation under external esophageal perfusion. / Li, Yan; Wo, John; Ellis, Susan; Ray, Mukunda B.; Jones, Whitney; Martin, Robert C G.

In: Stem Cells and Development, Vol. 15, No. 5, 10.2006, p. 697-705.

Research output: Contribution to journalArticle

Li, Yan ; Wo, John ; Ellis, Susan ; Ray, Mukunda B. ; Jones, Whitney ; Martin, Robert C G. / Morphological transformation in esophageal submucosa by bone marrow cells : Esophageal implantation under external esophageal perfusion. In: Stem Cells and Development. 2006 ; Vol. 15, No. 5. pp. 697-705.
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