Morphometric analysis of distinct microanatomy near the base of proximal tubule cells

L. W. Welling, D. J. Welling, J. W. Holsapple, Andrew Evan

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Citations (Scopus)

Abstract

Models of cell shape in the rabbit S2 proximal renal tubule were derived from transmission electron micrographs and compared with scanning micrographs. Standard morphometric procedures were used to measure basolateral cell membrane surface density (S(Vt)) relative to total epithelial volume in numerous zones of cell height. In the basal 20% region we also measured the volume fraction (F) of intercellular spaces and calculated new surface densities in reference only to the intercellular volume, S(Vi) = S(Vt)/F, or to the cellular volume, S(Vc) = S(Vt)/(1 - F). Combined use of these surface densities then enabled us to calculate the diameter, length, and separation of effectively cylindrical microvilli at the cell base. Assuming that lateral cell membranes are radially oriented in the apical region but disposed on microvillus like structures of arbitrary orientation at the cell base, an improved cell model was developed that agreed with the scanning picture throughout the entire cell height. Basal microvillar elements contain ~60% of the total basolateral cell membrane surface area and possibly constitute a hydrostatic resistive region for absorbate flow. These features have interesting physiological implications.

Original languageEnglish
Title of host publicationAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume253
Edition1
StatePublished - 1987
Externally publishedYes

Fingerprint

Cell Membrane
Microvilli
Proximal Kidney Tubule
Cell Shape
Extracellular Space
Electrons
Rabbits

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Welling, L. W., Welling, D. J., Holsapple, J. W., & Evan, A. (1987). Morphometric analysis of distinct microanatomy near the base of proximal tubule cells. In American Journal of Physiology - Renal Fluid and Electrolyte Physiology (1 ed., Vol. 253)

Morphometric analysis of distinct microanatomy near the base of proximal tubule cells. / Welling, L. W.; Welling, D. J.; Holsapple, J. W.; Evan, Andrew.

American Journal of Physiology - Renal Fluid and Electrolyte Physiology. Vol. 253 1. ed. 1987.

Research output: Chapter in Book/Report/Conference proceedingChapter

Welling, LW, Welling, DJ, Holsapple, JW & Evan, A 1987, Morphometric analysis of distinct microanatomy near the base of proximal tubule cells. in American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 1 edn, vol. 253.
Welling LW, Welling DJ, Holsapple JW, Evan A. Morphometric analysis of distinct microanatomy near the base of proximal tubule cells. In American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 1 ed. Vol. 253. 1987
Welling, L. W. ; Welling, D. J. ; Holsapple, J. W. ; Evan, Andrew. / Morphometric analysis of distinct microanatomy near the base of proximal tubule cells. American Journal of Physiology - Renal Fluid and Electrolyte Physiology. Vol. 253 1. ed. 1987.
@inbook{aa97ec4c096449ee9f274af9e87d9d91,
title = "Morphometric analysis of distinct microanatomy near the base of proximal tubule cells",
abstract = "Models of cell shape in the rabbit S2 proximal renal tubule were derived from transmission electron micrographs and compared with scanning micrographs. Standard morphometric procedures were used to measure basolateral cell membrane surface density (S(Vt)) relative to total epithelial volume in numerous zones of cell height. In the basal 20{\%} region we also measured the volume fraction (F) of intercellular spaces and calculated new surface densities in reference only to the intercellular volume, S(Vi) = S(Vt)/F, or to the cellular volume, S(Vc) = S(Vt)/(1 - F). Combined use of these surface densities then enabled us to calculate the diameter, length, and separation of effectively cylindrical microvilli at the cell base. Assuming that lateral cell membranes are radially oriented in the apical region but disposed on microvillus like structures of arbitrary orientation at the cell base, an improved cell model was developed that agreed with the scanning picture throughout the entire cell height. Basal microvillar elements contain ~60{\%} of the total basolateral cell membrane surface area and possibly constitute a hydrostatic resistive region for absorbate flow. These features have interesting physiological implications.",
author = "Welling, {L. W.} and Welling, {D. J.} and Holsapple, {J. W.} and Andrew Evan",
year = "1987",
language = "English",
volume = "253",
booktitle = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
edition = "1",

}

TY - CHAP

T1 - Morphometric analysis of distinct microanatomy near the base of proximal tubule cells

AU - Welling, L. W.

AU - Welling, D. J.

AU - Holsapple, J. W.

AU - Evan, Andrew

PY - 1987

Y1 - 1987

N2 - Models of cell shape in the rabbit S2 proximal renal tubule were derived from transmission electron micrographs and compared with scanning micrographs. Standard morphometric procedures were used to measure basolateral cell membrane surface density (S(Vt)) relative to total epithelial volume in numerous zones of cell height. In the basal 20% region we also measured the volume fraction (F) of intercellular spaces and calculated new surface densities in reference only to the intercellular volume, S(Vi) = S(Vt)/F, or to the cellular volume, S(Vc) = S(Vt)/(1 - F). Combined use of these surface densities then enabled us to calculate the diameter, length, and separation of effectively cylindrical microvilli at the cell base. Assuming that lateral cell membranes are radially oriented in the apical region but disposed on microvillus like structures of arbitrary orientation at the cell base, an improved cell model was developed that agreed with the scanning picture throughout the entire cell height. Basal microvillar elements contain ~60% of the total basolateral cell membrane surface area and possibly constitute a hydrostatic resistive region for absorbate flow. These features have interesting physiological implications.

AB - Models of cell shape in the rabbit S2 proximal renal tubule were derived from transmission electron micrographs and compared with scanning micrographs. Standard morphometric procedures were used to measure basolateral cell membrane surface density (S(Vt)) relative to total epithelial volume in numerous zones of cell height. In the basal 20% region we also measured the volume fraction (F) of intercellular spaces and calculated new surface densities in reference only to the intercellular volume, S(Vi) = S(Vt)/F, or to the cellular volume, S(Vc) = S(Vt)/(1 - F). Combined use of these surface densities then enabled us to calculate the diameter, length, and separation of effectively cylindrical microvilli at the cell base. Assuming that lateral cell membranes are radially oriented in the apical region but disposed on microvillus like structures of arbitrary orientation at the cell base, an improved cell model was developed that agreed with the scanning picture throughout the entire cell height. Basal microvillar elements contain ~60% of the total basolateral cell membrane surface area and possibly constitute a hydrostatic resistive region for absorbate flow. These features have interesting physiological implications.

UR - http://www.scopus.com/inward/record.url?scp=0023235532&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023235532&partnerID=8YFLogxK

M3 - Chapter

C2 - 3605343

AN - SCOPUS:0023235532

VL - 253

BT - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

ER -