Mouse and human islets survive and function after coating by biosilicification

David B. Jaroch, Jing Lu, Rajtarun Madangopal, Natalie D. Stull, Matthew Stensberg, Jin Shi, Jennifer L. Kahn, Ruth Herrera-Perez, Michael Zeitchek, Jennifer Sturgis, J. Paul Robinson, Mervin C. Yoder, D. Marshall Porterfield, Raghavendra G. Mirmira, Jenna L. Rickus

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Inorganic materials have properties that can be advantageous in bioencapsulation for cell transplantation. Our aim was to engineer a hybrid inorganic/soft tissue construct by inducing pancreatic islets to grow an inorganic shell. We created pancreatic islets surrounded by porous silica, which has potential application in the immunoprotection of islets in transplantation therapies for type 1 diabetes. The new method takes advantage of the islet capsule surface as a template for silica formation. Mouse and human islets were exposed to medium containing saturating silicic acid levels for 9-15 min. The resulting tissue constructs were then cultured for up to 4 wk under normal conditions. Scanning electron microscopy and energy dispersive X-ray spectroscopy was used to monitor the morphology and elemental composition of the material at the islet surface. A cytokine assay was used to assess biocompatibility with macrophages. Islet survival and function were assessed by confocal microscopy, glucose-stimulated insulin release assays, oxygen flux at the islet surface, expression of key genes by RT-PCR, and syngeneic transplant into diabetic mice.

Original languageEnglish (US)
Pages (from-to)E1230-E1240
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume305
Issue number10
DOIs
StatePublished - Nov 15 2013

Fingerprint

Islets of Langerhans
Silicon Dioxide
Silicic Acid
X-Ray Emission Spectrometry
Islets of Langerhans Transplantation
Cell Transplantation
Type 1 Diabetes Mellitus
Confocal Microscopy
Electron Scanning Microscopy
Capsules
Macrophages
Insulin
Cytokines
Oxygen
Transplants
Gene Expression
Glucose
Polymerase Chain Reaction
Survival
Therapeutics

Keywords

  • Coating
  • Encapsulation
  • Islet
  • Silica
  • Tissue engineering

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Endocrinology, Diabetes and Metabolism

Cite this

Jaroch, D. B., Lu, J., Madangopal, R., Stull, N. D., Stensberg, M., Shi, J., ... Rickus, J. L. (2013). Mouse and human islets survive and function after coating by biosilicification. American Journal of Physiology - Endocrinology and Metabolism, 305(10), E1230-E1240. https://doi.org/10.1152/ajpendo.00081.2013

Mouse and human islets survive and function after coating by biosilicification. / Jaroch, David B.; Lu, Jing; Madangopal, Rajtarun; Stull, Natalie D.; Stensberg, Matthew; Shi, Jin; Kahn, Jennifer L.; Herrera-Perez, Ruth; Zeitchek, Michael; Sturgis, Jennifer; Robinson, J. Paul; Yoder, Mervin C.; Porterfield, D. Marshall; Mirmira, Raghavendra G.; Rickus, Jenna L.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 305, No. 10, 15.11.2013, p. E1230-E1240.

Research output: Contribution to journalArticle

Jaroch, DB, Lu, J, Madangopal, R, Stull, ND, Stensberg, M, Shi, J, Kahn, JL, Herrera-Perez, R, Zeitchek, M, Sturgis, J, Robinson, JP, Yoder, MC, Porterfield, DM, Mirmira, RG & Rickus, JL 2013, 'Mouse and human islets survive and function after coating by biosilicification', American Journal of Physiology - Endocrinology and Metabolism, vol. 305, no. 10, pp. E1230-E1240. https://doi.org/10.1152/ajpendo.00081.2013
Jaroch, David B. ; Lu, Jing ; Madangopal, Rajtarun ; Stull, Natalie D. ; Stensberg, Matthew ; Shi, Jin ; Kahn, Jennifer L. ; Herrera-Perez, Ruth ; Zeitchek, Michael ; Sturgis, Jennifer ; Robinson, J. Paul ; Yoder, Mervin C. ; Porterfield, D. Marshall ; Mirmira, Raghavendra G. ; Rickus, Jenna L. / Mouse and human islets survive and function after coating by biosilicification. In: American Journal of Physiology - Endocrinology and Metabolism. 2013 ; Vol. 305, No. 10. pp. E1230-E1240.
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