Mouse and human islets survive and function after coating by biosilicification

David B. Jaroch, Jing Lu, Rajtarun Madangopal, Natalie D. Stull, Matthew Stensberg, Jin Shi, Jennifer L. Kahn, Ruth Herrera-Perez, Michael Zeitchek, Jennifer Sturgis, J. Paul Robinson, Mervin C. Yoder, D. Marshall Porterfield, Raghavendra G. Mirmira, Jenna L. Rickus

Research output: Contribution to journalArticle

13 Scopus citations


Inorganic materials have properties that can be advantageous in bioencapsulation for cell transplantation. Our aim was to engineer a hybrid inorganic/soft tissue construct by inducing pancreatic islets to grow an inorganic shell. We created pancreatic islets surrounded by porous silica, which has potential application in the immunoprotection of islets in transplantation therapies for type 1 diabetes. The new method takes advantage of the islet capsule surface as a template for silica formation. Mouse and human islets were exposed to medium containing saturating silicic acid levels for 9-15 min. The resulting tissue constructs were then cultured for up to 4 wk under normal conditions. Scanning electron microscopy and energy dispersive X-ray spectroscopy was used to monitor the morphology and elemental composition of the material at the islet surface. A cytokine assay was used to assess biocompatibility with macrophages. Islet survival and function were assessed by confocal microscopy, glucose-stimulated insulin release assays, oxygen flux at the islet surface, expression of key genes by RT-PCR, and syngeneic transplant into diabetic mice.

Original languageEnglish (US)
Pages (from-to)E1230-E1240
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Issue number10
StatePublished - Nov 15 2013


  • Coating
  • Encapsulation
  • Islet
  • Silica
  • Tissue engineering

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Endocrinology, Diabetes and Metabolism

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  • Cite this

    Jaroch, D. B., Lu, J., Madangopal, R., Stull, N. D., Stensberg, M., Shi, J., Kahn, J. L., Herrera-Perez, R., Zeitchek, M., Sturgis, J., Robinson, J. P., Yoder, M. C., Porterfield, D. M., Mirmira, R. G., & Rickus, J. L. (2013). Mouse and human islets survive and function after coating by biosilicification. American Journal of Physiology - Endocrinology and Metabolism, 305(10), E1230-E1240.