Mouse Hind Limb Skeletal Muscle Functional Adaptation in a Simulated Fine Branch Arboreal Habitat

Joseph E. Rupert, J. Ethan Joll, Wiaam Y. Elkhatib, Jason Organ

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The musculoskeletal system is remarkably plastic during growth. The purpose of this study was to examine the muscular plasticity in functional and structural properties in a model known to result in significant developmental plasticity of the postcranial skeleton. Fifteen weanling C57BL/6 mice were raised to 16 weeks of age in one of two enclosures: a climbing enclosure that simulates a fine branch arboreal habitat and is traversed by steel wires crossing at 45° relative to horizontal at multiple intersections, and a control enclosure that resembles a parking deck with no wires but the same volume of habitable space. At killing, ex vivo contractility properties of the soleus (SOL) and extensor digitorum longus (EDL) muscles were examined. Our results demonstrate that EDL muscles of climbing mice contracted with higher specific forces and were comprised of muscle fibers with slower myosin heavy chain isoforms. EDL muscles also fatigued at a higher rate in climbing mice compared to controls. SOL muscle function is not affected by the climbing environment. Likewise, mass and architecture of both EDL and SOL muscles were not different between climbing and control mice. Our data demonstrate that functional adaptation does not require concomitant architectural adaptation in order to increase contractile force. Anat Rec, 301:434–440, 2018.

Original languageEnglish (US)
Pages (from-to)434-440
Number of pages7
JournalAnatomical Record
Volume301
Issue number3
DOIs
StatePublished - Mar 1 2018

Fingerprint

limbs (animal)
branches
Ecosystem
skeletal muscle
limb
Skeletal Muscle
muscle
Extremities
Muscles
muscles
mice
habitat
habitats
wire
Musculoskeletal System
plasticity
Myosin Heavy Chains
Steel
musculoskeletal system
Inbred C57BL Mouse

Keywords

  • climbing
  • ex vivo contractility
  • fatigue
  • specific force

ASJC Scopus subject areas

  • Anatomy
  • Biotechnology
  • Histology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Mouse Hind Limb Skeletal Muscle Functional Adaptation in a Simulated Fine Branch Arboreal Habitat. / Rupert, Joseph E.; Joll, J. Ethan; Elkhatib, Wiaam Y.; Organ, Jason.

In: Anatomical Record, Vol. 301, No. 3, 01.03.2018, p. 434-440.

Research output: Contribution to journalArticle

Rupert, Joseph E. ; Joll, J. Ethan ; Elkhatib, Wiaam Y. ; Organ, Jason. / Mouse Hind Limb Skeletal Muscle Functional Adaptation in a Simulated Fine Branch Arboreal Habitat. In: Anatomical Record. 2018 ; Vol. 301, No. 3. pp. 434-440.
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