Mouse placental 57-kDa calcium-binding protein: II. Localization of mRNA in mouse and human placentae by in situ cDNA hybridization

Rocky S. Tuan, Bruce T. Lamb, Claire B. Jesinkey

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Using a cloned cDNA to the mouse placental calcium-binding protein (MCaBP) [Tuan and Kirwin, preceding paper], mRNA transcripts of the MCaBP have been localized in the mouse placenta by in situ cDNA-RNA hybridization. The procedure involved the use of a nonradioactive, biotinylated cDNA probe. Paraffin sections of the mouse chorioallantoic placenta were processed for in situ hybridization using a procedure modified from that of Lo [8]. The results demonstrated that MCaBP mRNA is localized specifically to the fetal trophoblast of the mouse placenta, and is expressed in a development-dependent manner. The MCaBP cDNA also hybridized strongly in situ to sections of human term placenta, giving rise to signals localized specifically to the syncytiotrophoblastic layer of the chorionic villi.

Original languageEnglish (US)
Pages (from-to)198-204
Number of pages7
JournalDifferentiation
Volume37
Issue number3
DOIs
StatePublished - Jan 1 1988

Fingerprint

Placenta
In Situ Hybridization
Complementary DNA
Messenger RNA
Chorionic Villi
Trophoblasts
57-kDa calcium-binding protein
Paraffin
RNA
S100 Calcium-Binding Protein A4

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology
  • Cancer Research

Cite this

Mouse placental 57-kDa calcium-binding protein : II. Localization of mRNA in mouse and human placentae by in situ cDNA hybridization. / Tuan, Rocky S.; Lamb, Bruce T.; Jesinkey, Claire B.

In: Differentiation, Vol. 37, No. 3, 01.01.1988, p. 198-204.

Research output: Contribution to journalArticle

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