Mouse RAD50 has limited epitopic homology to p53 and is expressed in the adult myocardium

Kyung Keun Kim, Adil I. Daud, Susan C. Wong, Laura Pajak, Shih Chong Tsai, He Wang, William J. Henzel, Loren J. Field

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Previous studies have identified a 180-kDa mouse cardiomyocyte phosphoprotein with limited epitopic homology to p53. In this study, the protein was purified and partially sequenced. Oligonucleotide probes based on the available amino acid sequence data were used to isolate cDNA clones. Sequence analyses revealed that the clones encoded a protein with regional homology to the yeast RAD50 gene product. Expression of the mouse cDNA rescued the methyl methanesulfonate-sensitive phenotype in rad50 mutant yeast, indicating that the cardiomyocyte phosphoprotein is the mammalian homologue of the yeast RAD50 gene product. Fluorescence in situ hybridization analyses localized the mouse RAD50 gene to the A5-B1 region of chromosome 11. Northern blot analyses demonstrated a complex pattern of RAD50 expression during mouse development which was further complicated by the presence of several alternatively spliced transcripts. High levels of RAD50 expression was evident in the adult myocardium, a somewhat surprising observation given the absence of DNA synthesis in adult cardiomyocytes.

Original languageEnglish (US)
Pages (from-to)29255-29264
Number of pages10
JournalJournal of Biological Chemistry
Volume271
Issue number46
DOIs
StatePublished - 1996

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Kim, K. K., Daud, A. I., Wong, S. C., Pajak, L., Tsai, S. C., Wang, H., Henzel, W. J., & Field, L. J. (1996). Mouse RAD50 has limited epitopic homology to p53 and is expressed in the adult myocardium. Journal of Biological Chemistry, 271(46), 29255-29264. https://doi.org/10.1074/jbc.271.46.29255