Mouse SLLP1, a sperm lysozyme-like protein involved in sperm-egg binding and fertilization

María Belén Herrero, Arabinda Mandal, Laura C. Digilio, Scott A. Coonrod, Bernhard Maier, John C. Herr

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

This study demonstrates the retention of mouse sperm lysozyme-like protein (mSLLP1) in the equatorial segment of spermatozoa following the acrosome reaction and a role for mSLLP1 in sperm-egg binding and fertilization. Treatment of cumulus intact oocytes with either recmSLLP1 or its antiserum resulted in a significant (P ≤ 0.05) inhibition of fertilization. Co-incubation of zona-free mouse oocytes with capacitated mouse spermatozoa in the presence of varying concentrations of anti-recmSLLP1 serum or recmSLLP1 also inhibited sperm-oolemma binding. A complete inhibition of binding and fusion of spermatozoa to the oocyte occurred at 12.5 μM concentration of recmSLLP1, while conventional chicken and human lysozymes did not block sperm-egg binding. mSLLP1 showed receptor sites in the perivitelline space as well as on the microvillar region of the egg plasma membrane. The retention of mSLLP1 in the equatorial segment of acrosome-reacted sperm, the inhibitory effects of both recmSLLP1 and antibodies to SLLP1 on in vitro fertilization with both cumulus intact and zona-free eggs, and the definition of complementary SLLP1-binding sites on the egg plasma membrane together support the hypothesis that a c lysozyme-like protein is involved in the binding of spermatozoa to the egg plasma membrane during fertilization.

Original languageEnglish (US)
Pages (from-to)126-142
Number of pages17
JournalDevelopmental Biology
Volume284
Issue number1
DOIs
StatePublished - Aug 1 2005
Externally publishedYes

Fingerprint

Muramidase
Fertilization
Ovum
Spermatozoa
Proteins
Oocytes
Cell Membrane
Herpes Zoster
Acrosome Reaction
Acrosome
Fertilization in Vitro
Contraception
Eggs
Immune Sera
Chickens
Binding Sites
Antibodies
Serum

Keywords

  • Acrosome
  • Fertilization
  • Lysozyme-like
  • Mouse
  • Perivitelline matrix
  • SLLP1
  • Sperm-egg binding
  • Sperm-egg fusion
  • Spermatozoa

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Mouse SLLP1, a sperm lysozyme-like protein involved in sperm-egg binding and fertilization. / Herrero, María Belén; Mandal, Arabinda; Digilio, Laura C.; Coonrod, Scott A.; Maier, Bernhard; Herr, John C.

In: Developmental Biology, Vol. 284, No. 1, 01.08.2005, p. 126-142.

Research output: Contribution to journalArticle

Herrero, María Belén ; Mandal, Arabinda ; Digilio, Laura C. ; Coonrod, Scott A. ; Maier, Bernhard ; Herr, John C. / Mouse SLLP1, a sperm lysozyme-like protein involved in sperm-egg binding and fertilization. In: Developmental Biology. 2005 ; Vol. 284, No. 1. pp. 126-142.
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