MRI of fat distribution in a mouse model of lysosomal acid lipase deficiency

Hong Du, Bernard J. Dardzinski, Kendall J. O'Brien, Lane F. Donnelly

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

OBJECTIVE. We assessed the use of MRI in the evaluation of abdominal fat distribution in a lysosomal acid lipase (LAL)-deficient mouse model. MATERIALS AND METHODS. LAL-deficient mice are born with a normal fat distribution but over time deplete the fat stores in the subcutaneous and retroperitoneal tissues and accumulate fat in the liver, spleen, and bowel. Four MRI studies of LAL-deficient mice and control mice were obtained with 3-T T1-weighted spin-echo images and volume segmentation processing to create parameters for the study of fat distribution: intraabdominal adipose tissue-subcutaneous adipose tissue (IAT/SAT) ratio, liver volume, reproductive fat, and retroperitoneal fat. MRI adiposity parameters in LAL-deficient mice were compared with those in control mice. Adiposity volumes calculated on MRI were compared with those calculated at autopsy. RESULTS. Statistically significant differences were found between LAL-deficient and control mice for IAT/SAT ratio (p = 0.0336), liver volume (p = 0.0336), and reproductive fat (p = 0.0336), and a statistically significant trend was found for retroperitoneal fat (p = 0.0514). No statistically significant difference was found between adiposity volumes calculated on MRI and adiposity volumes found at autopsy (all p > 0.2). CONCLUSION. Use of an in vivo model showed MRI techniques to be accurate in predicting visceral adiposity. LAL-deficient mice provided a unique model showing a pattern of adipose distribution that is markedly different from that in control mice, and MRI may provide a means of evaluating therapeutic interventions sequentially.

Original languageEnglish (US)
Pages (from-to)658-662
Number of pages5
JournalAmerican Journal of Roentgenology
Volume184
Issue number2
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

Fingerprint

Adiposity
Fats
Intra-Abdominal Fat
Subcutaneous Fat
Adipose Tissue
Autopsy
Liver
Sterol Esterase
Abdominal Fat
Normal Distribution
Subcutaneous Tissue
Lysosomal acid lipase deficiency
mouse lysosomal acid lipase
Spleen

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

MRI of fat distribution in a mouse model of lysosomal acid lipase deficiency. / Du, Hong; Dardzinski, Bernard J.; O'Brien, Kendall J.; Donnelly, Lane F.

In: American Journal of Roentgenology, Vol. 184, No. 2, 01.01.2005, p. 658-662.

Research output: Contribution to journalArticle

Du, Hong ; Dardzinski, Bernard J. ; O'Brien, Kendall J. ; Donnelly, Lane F. / MRI of fat distribution in a mouse model of lysosomal acid lipase deficiency. In: American Journal of Roentgenology. 2005 ; Vol. 184, No. 2. pp. 658-662.
@article{7db44c2cf36a470fbea9091f62ee9322,
title = "MRI of fat distribution in a mouse model of lysosomal acid lipase deficiency",
abstract = "OBJECTIVE. We assessed the use of MRI in the evaluation of abdominal fat distribution in a lysosomal acid lipase (LAL)-deficient mouse model. MATERIALS AND METHODS. LAL-deficient mice are born with a normal fat distribution but over time deplete the fat stores in the subcutaneous and retroperitoneal tissues and accumulate fat in the liver, spleen, and bowel. Four MRI studies of LAL-deficient mice and control mice were obtained with 3-T T1-weighted spin-echo images and volume segmentation processing to create parameters for the study of fat distribution: intraabdominal adipose tissue-subcutaneous adipose tissue (IAT/SAT) ratio, liver volume, reproductive fat, and retroperitoneal fat. MRI adiposity parameters in LAL-deficient mice were compared with those in control mice. Adiposity volumes calculated on MRI were compared with those calculated at autopsy. RESULTS. Statistically significant differences were found between LAL-deficient and control mice for IAT/SAT ratio (p = 0.0336), liver volume (p = 0.0336), and reproductive fat (p = 0.0336), and a statistically significant trend was found for retroperitoneal fat (p = 0.0514). No statistically significant difference was found between adiposity volumes calculated on MRI and adiposity volumes found at autopsy (all p > 0.2). CONCLUSION. Use of an in vivo model showed MRI techniques to be accurate in predicting visceral adiposity. LAL-deficient mice provided a unique model showing a pattern of adipose distribution that is markedly different from that in control mice, and MRI may provide a means of evaluating therapeutic interventions sequentially.",
author = "Hong Du and Dardzinski, {Bernard J.} and O'Brien, {Kendall J.} and Donnelly, {Lane F.}",
year = "2005",
month = "1",
day = "1",
doi = "10.2214/ajr.184.2.01840658",
language = "English (US)",
volume = "184",
pages = "658--662",
journal = "American Journal of Roentgenology",
issn = "0361-803X",
publisher = "American Roentgen Ray Society",
number = "2",

}

TY - JOUR

T1 - MRI of fat distribution in a mouse model of lysosomal acid lipase deficiency

AU - Du, Hong

AU - Dardzinski, Bernard J.

AU - O'Brien, Kendall J.

AU - Donnelly, Lane F.

PY - 2005/1/1

Y1 - 2005/1/1

N2 - OBJECTIVE. We assessed the use of MRI in the evaluation of abdominal fat distribution in a lysosomal acid lipase (LAL)-deficient mouse model. MATERIALS AND METHODS. LAL-deficient mice are born with a normal fat distribution but over time deplete the fat stores in the subcutaneous and retroperitoneal tissues and accumulate fat in the liver, spleen, and bowel. Four MRI studies of LAL-deficient mice and control mice were obtained with 3-T T1-weighted spin-echo images and volume segmentation processing to create parameters for the study of fat distribution: intraabdominal adipose tissue-subcutaneous adipose tissue (IAT/SAT) ratio, liver volume, reproductive fat, and retroperitoneal fat. MRI adiposity parameters in LAL-deficient mice were compared with those in control mice. Adiposity volumes calculated on MRI were compared with those calculated at autopsy. RESULTS. Statistically significant differences were found between LAL-deficient and control mice for IAT/SAT ratio (p = 0.0336), liver volume (p = 0.0336), and reproductive fat (p = 0.0336), and a statistically significant trend was found for retroperitoneal fat (p = 0.0514). No statistically significant difference was found between adiposity volumes calculated on MRI and adiposity volumes found at autopsy (all p > 0.2). CONCLUSION. Use of an in vivo model showed MRI techniques to be accurate in predicting visceral adiposity. LAL-deficient mice provided a unique model showing a pattern of adipose distribution that is markedly different from that in control mice, and MRI may provide a means of evaluating therapeutic interventions sequentially.

AB - OBJECTIVE. We assessed the use of MRI in the evaluation of abdominal fat distribution in a lysosomal acid lipase (LAL)-deficient mouse model. MATERIALS AND METHODS. LAL-deficient mice are born with a normal fat distribution but over time deplete the fat stores in the subcutaneous and retroperitoneal tissues and accumulate fat in the liver, spleen, and bowel. Four MRI studies of LAL-deficient mice and control mice were obtained with 3-T T1-weighted spin-echo images and volume segmentation processing to create parameters for the study of fat distribution: intraabdominal adipose tissue-subcutaneous adipose tissue (IAT/SAT) ratio, liver volume, reproductive fat, and retroperitoneal fat. MRI adiposity parameters in LAL-deficient mice were compared with those in control mice. Adiposity volumes calculated on MRI were compared with those calculated at autopsy. RESULTS. Statistically significant differences were found between LAL-deficient and control mice for IAT/SAT ratio (p = 0.0336), liver volume (p = 0.0336), and reproductive fat (p = 0.0336), and a statistically significant trend was found for retroperitoneal fat (p = 0.0514). No statistically significant difference was found between adiposity volumes calculated on MRI and adiposity volumes found at autopsy (all p > 0.2). CONCLUSION. Use of an in vivo model showed MRI techniques to be accurate in predicting visceral adiposity. LAL-deficient mice provided a unique model showing a pattern of adipose distribution that is markedly different from that in control mice, and MRI may provide a means of evaluating therapeutic interventions sequentially.

UR - http://www.scopus.com/inward/record.url?scp=15944389534&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=15944389534&partnerID=8YFLogxK

U2 - 10.2214/ajr.184.2.01840658

DO - 10.2214/ajr.184.2.01840658

M3 - Article

VL - 184

SP - 658

EP - 662

JO - American Journal of Roentgenology

JF - American Journal of Roentgenology

SN - 0361-803X

IS - 2

ER -