Mucosal administration of collagen V ameliorates the atherosclerotic plaque burden by inducing interleukin 35-dependent tolerance

Arick C. Park, Guorui Huang, Ewa Jankowska-Gan, Dawiyat Massoudi, John F. Kernien, Dario A. Vignali, Jeremy A. Sullivan, David S. Wilkes, William J. Burlingham, Daniel S. Greenspan

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We have shown previously that collagen V (col(V)) autoimmunity is a consistent feature of atherosclerosis in human coronary artery disease and in the Apoe-/- mouse model. We have also shown sensitization of Apoe-/-mice with col(V) to markedly increase the atherosclerotic burden, providing evidence of a causative role for col(V) autoimmunity in atherosclerotic pathogenesis. Here we sought to determine whether induction of immune tolerance to col(V) might ameliorate atherosclerosis, providing further evidence for a causal role for col(V) autoimmunity in atherogenesis and providing insights into the potential for immunomodulatory therapeutic interventions. Mucosal inoculation successfully inducedimmunetolerance to col(V) with an accompanying reduction in plaque burden in Ldlr-/- mice on a high-cholesterol diet. The results therefore demonstrate that inoculation with col(V) can successfully ameliorate the atherosclerotic burden, suggesting novel approaches for therapeutic interventions. Surprisingly, tolerance and reduced atherosclerotic burden were both dependent on the recently described IL-35 and not on IL-10, the immunosuppressive cytokine usually studied in the context of induced tolerance and amelioration of atherosclerotic symptoms. In addition to the above, using recombinant protein fragments, we were able to localize two epitopes of the α1(V) chain involved in col(V) autoimmunity in atherosclerotic Ldlr-/- mice, suggesting future courses of experimentation for the characterization of such epitopes.

Original languageEnglish (US)
Pages (from-to)3359-3370
Number of pages12
JournalJournal of Biological Chemistry
Volume291
Issue number7
DOIs
StatePublished - Feb 12 2016

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Park, A. C., Huang, G., Jankowska-Gan, E., Massoudi, D., Kernien, J. F., Vignali, D. A., Sullivan, J. A., Wilkes, D. S., Burlingham, W. J., & Greenspan, D. S. (2016). Mucosal administration of collagen V ameliorates the atherosclerotic plaque burden by inducing interleukin 35-dependent tolerance. Journal of Biological Chemistry, 291(7), 3359-3370. https://doi.org/10.1074/jbc.M115.681882