Multi-center evaluation of analytical performance of the Beckman Coulter AU5822 chemistry analyzer

Michelle Zimmerman, L. R. Friesen, A. Nice, P. A. Vollmer, E. A. Dockery, J. D. Rankin, K. Zmuda, S. H. Wong

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objectives: Our three academic institutions, Indiana University, Northwestern Memorial Hospital, and Wake Forest, were among the first in the United States to implement the Beckman Coulter AU5822 series chemistry analyzers. We undertook this post-hoc multi-center study by merging our data to determine performance characteristics and the impact of methodology changes on analyte measurement. Design and methods: We independently completed performance validation studies including precision, linearity/analytical measurement range, method comparison, and reference range verification. Complete data sets were available from at least one institution for 66 analytes with the following groups: 51 from all three institutions, and 15 from 1 or 2 institutions for a total sample size of 12,064. Results: Precision was similar among institutions. Coefficients of variation (CV) were <. 10% for 97%. Analytes with CVs >. 10% included direct bilirubin and digoxin. All analytes exhibited linearity over the analytical measurement range. Method comparison data showed slopes between 0.900-1.100 for 87.9% of the analytes. Slopes for amylase, tobramycin and urine amylase were <. 0.8; the slope for lipase was >. 1.5, due to known methodology or standardization differences. Consequently, reference ranges of amylase, urine amylase and lipase required only minor or no modification. Conclusion: The four AU5822 analyzers independently evaluated at three sites showed consistent precision, linearity, and correlation results. Since installations, the test results had been well received by clinicians from all three institutions.

Original languageEnglish (US)
Pages (from-to)881-885
Number of pages5
JournalClinical Biochemistry
Volume48
Issue number13-14
DOIs
StatePublished - Sep 1 2015

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Amylases
Reference Values
Urine
Tobramycin
Validation Studies
Digoxin
Lipase
Bilirubin
Sample Size
Merging
Standardization

Keywords

  • AU chemistry analyzer
  • Multi-center study
  • Performance evaluation
  • TDM and toxicology analyzers

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Medicine(all)

Cite this

Zimmerman, M., Friesen, L. R., Nice, A., Vollmer, P. A., Dockery, E. A., Rankin, J. D., ... Wong, S. H. (2015). Multi-center evaluation of analytical performance of the Beckman Coulter AU5822 chemistry analyzer. Clinical Biochemistry, 48(13-14), 881-885. https://doi.org/10.1016/j.clinbiochem.2015.06.010

Multi-center evaluation of analytical performance of the Beckman Coulter AU5822 chemistry analyzer. / Zimmerman, Michelle; Friesen, L. R.; Nice, A.; Vollmer, P. A.; Dockery, E. A.; Rankin, J. D.; Zmuda, K.; Wong, S. H.

In: Clinical Biochemistry, Vol. 48, No. 13-14, 01.09.2015, p. 881-885.

Research output: Contribution to journalArticle

Zimmerman, M, Friesen, LR, Nice, A, Vollmer, PA, Dockery, EA, Rankin, JD, Zmuda, K & Wong, SH 2015, 'Multi-center evaluation of analytical performance of the Beckman Coulter AU5822 chemistry analyzer', Clinical Biochemistry, vol. 48, no. 13-14, pp. 881-885. https://doi.org/10.1016/j.clinbiochem.2015.06.010
Zimmerman, Michelle ; Friesen, L. R. ; Nice, A. ; Vollmer, P. A. ; Dockery, E. A. ; Rankin, J. D. ; Zmuda, K. ; Wong, S. H. / Multi-center evaluation of analytical performance of the Beckman Coulter AU5822 chemistry analyzer. In: Clinical Biochemistry. 2015 ; Vol. 48, No. 13-14. pp. 881-885.
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