Multi-dimensional liquid chromatography in proteomics-A review

X. Zhang, Aiqin Fang, Catherine P. Riley, Mu Wang, Fred E. Regnier, Charles Buck

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Proteomics is the large-scale study of proteins, particularly their expression, structures and functions. This still-emerging combination of technologies aims to describe and characterize all expressed proteins in a biological system. Because of upper limits on mass detection of mass spectrometers, proteins are usually digested into peptides and the peptides are then separated, identified and quantified from this complex enzymatic digest. The problem in digesting proteins first and then analyzing the peptide cleavage fragments by mass spectrometry is that huge numbers of peptides are generated that overwhelm direct mass spectral analyses. The objective in the liquid chromatography approach to proteomics is to fractionate peptide mixtures to enable and maximize identification and quantification of the component peptides by mass spectrometry. This review will focus on existing multidimensional liquid chromatographic (MDLC) platforms developed for proteomics and their application in combination with other techniques such as stable isotope labeling. We also provide some perspectives on likely future developments.

Original languageEnglish
Pages (from-to)101-113
Number of pages13
JournalAnalytica Chimica Acta
Volume664
Issue number2
DOIs
StatePublished - Apr 2010

Fingerprint

proteomics
Liquid chromatography
Liquid Chromatography
peptide
Proteomics
liquid chromatography
Peptides
protein
Mass Spectrometry
Proteins
Mass spectrometry
Isotope Labeling
mass spectrometry
Peptide Fragments
Mass spectrometers
Biological systems
Isotopes
Labeling
cleavage
Technology

Keywords

  • Label-free
  • Multi-dimensional liquid chromatography
  • Proteomics
  • Stable isotope labeling

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Zhang, X., Fang, A., Riley, C. P., Wang, M., Regnier, F. E., & Buck, C. (2010). Multi-dimensional liquid chromatography in proteomics-A review. Analytica Chimica Acta, 664(2), 101-113. https://doi.org/10.1016/j.aca.2010.02.001

Multi-dimensional liquid chromatography in proteomics-A review. / Zhang, X.; Fang, Aiqin; Riley, Catherine P.; Wang, Mu; Regnier, Fred E.; Buck, Charles.

In: Analytica Chimica Acta, Vol. 664, No. 2, 04.2010, p. 101-113.

Research output: Contribution to journalArticle

Zhang, X, Fang, A, Riley, CP, Wang, M, Regnier, FE & Buck, C 2010, 'Multi-dimensional liquid chromatography in proteomics-A review', Analytica Chimica Acta, vol. 664, no. 2, pp. 101-113. https://doi.org/10.1016/j.aca.2010.02.001
Zhang, X. ; Fang, Aiqin ; Riley, Catherine P. ; Wang, Mu ; Regnier, Fred E. ; Buck, Charles. / Multi-dimensional liquid chromatography in proteomics-A review. In: Analytica Chimica Acta. 2010 ; Vol. 664, No. 2. pp. 101-113.
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