Multi-walled carbon nanotube directed gene and protein expression in cultured human aortic endothelial cells is influenced by suspension medium

Achini K. Vidanapathirana, Xianyin Lai, Susana C. Hilderbrand, Josh E. Pitzer, Ramakrishna Podila, Susan J. Sumner, Timothy R. Fennell, Christopher J. Wingard, Frank Witzmann, Jared M. Brown

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The use and production of multi-walled carbon nanotubes (MWCNTs) have significantly increased over the last decade due to their versatility in numerous applications. Their unique physical and chemical properties make them desirable for various biomedical applications, but the same properties also raise concerns about their safety to human health, particularly at the cellular level. The vascular endothelium could be exposed to nanomaterials either by direct intravenous administration in nanomedicine or by translocation following inhalational exposure in an occupational setting. We hypothesized that direct exposure to MWCNTs will increase the expression of inflammatory markers in human aortic endothelial cells (HAEC). We also investigated the effect of the route of exposure on activation by changing the suspension medium of the MWCNTs. HAEC were treated in vitro with MWCNTs (1 or 10 μg/cm2) suspended in either cell culture medium [(M)-MWCNTs] or 10% clinical grade pulmonary surfactant [(S)-MWCNTs]. The zeta potential of the (S)-MWCNTs was significantly more negative than the (M)-MWCNTs suggesting a more stable suspension. Treatment of HAEC with (S)-MWCNTs; as compared to (M)-MWCNTs resulted in a significantly higher up-regulation of mRNA transcripts for cell adhesion molecules VCAM1, SELE, ICAM1 and the chemokine CCL2. Time dependent changes in VCAM1 and CCL2 protein levels were confirmed by immunofluorescence, flow cytometry and ELISA. A label free quantitative mass spectrometry proteomic analysis was utilized to compare protein expression patterns between the two suspensions of MWCNTs. We identified significant expression changes in >200 unique proteins in MWCNT treated HAEC. However, the two suspensions of MWCNTs resulted in different protein expression patterns with the eIF2 pathway as the only common pathway identified between the two suspensions. These data suggest that direct exposure to MWCNTs induces acute inflammatory and protein expression changes in HAEC, which is influenced by the type of media used for suspension of MWCNTs and their resulting zeta potential.

Original languageEnglish
Pages (from-to)114-122
Number of pages9
JournalToxicology
Volume302
Issue number2-3
DOIs
StatePublished - 2012

Fingerprint

Carbon Nanotubes
Endothelial cells
Suspensions
Endothelial Cells
Gene Expression
Proteins
Zeta potential
Nanomedicine
Medical nanotechnology
Pulmonary Surfactants
Flow cytometry
Nanostructures
Chemokine CCL2
Vascular Endothelium
Cell Adhesion Molecules
Occupational Exposure
Cell culture
Nanostructured materials
Intravenous Administration
Proteomics

Keywords

  • eiF2
  • MWCNT
  • Nanomedicine
  • Nanotoxicology
  • Protein corona
  • Surfactant

ASJC Scopus subject areas

  • Toxicology

Cite this

Vidanapathirana, A. K., Lai, X., Hilderbrand, S. C., Pitzer, J. E., Podila, R., Sumner, S. J., ... Brown, J. M. (2012). Multi-walled carbon nanotube directed gene and protein expression in cultured human aortic endothelial cells is influenced by suspension medium. Toxicology, 302(2-3), 114-122. https://doi.org/10.1016/j.tox.2012.09.008

Multi-walled carbon nanotube directed gene and protein expression in cultured human aortic endothelial cells is influenced by suspension medium. / Vidanapathirana, Achini K.; Lai, Xianyin; Hilderbrand, Susana C.; Pitzer, Josh E.; Podila, Ramakrishna; Sumner, Susan J.; Fennell, Timothy R.; Wingard, Christopher J.; Witzmann, Frank; Brown, Jared M.

In: Toxicology, Vol. 302, No. 2-3, 2012, p. 114-122.

Research output: Contribution to journalArticle

Vidanapathirana, AK, Lai, X, Hilderbrand, SC, Pitzer, JE, Podila, R, Sumner, SJ, Fennell, TR, Wingard, CJ, Witzmann, F & Brown, JM 2012, 'Multi-walled carbon nanotube directed gene and protein expression in cultured human aortic endothelial cells is influenced by suspension medium', Toxicology, vol. 302, no. 2-3, pp. 114-122. https://doi.org/10.1016/j.tox.2012.09.008
Vidanapathirana, Achini K. ; Lai, Xianyin ; Hilderbrand, Susana C. ; Pitzer, Josh E. ; Podila, Ramakrishna ; Sumner, Susan J. ; Fennell, Timothy R. ; Wingard, Christopher J. ; Witzmann, Frank ; Brown, Jared M. / Multi-walled carbon nanotube directed gene and protein expression in cultured human aortic endothelial cells is influenced by suspension medium. In: Toxicology. 2012 ; Vol. 302, No. 2-3. pp. 114-122.
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