MultiBac: Expanding the research toolbox for multiprotein complexes

Christoph Bieniossek, Tsuyoshi Imasaki, Yuichiro Takagi, Imre Berger

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

Protein complexes composed of many subunits carry out most essential processes in cells and, therefore, have become the focus of intense research. However, deciphering the structure and function of these multiprotein assemblies imposes the challenging task of producing them in sufficient quality and quantity. To overcome this bottleneck, powerful recombinant expression technologies are being developed. In this review, we describe the use of one of these technologies, MultiBac, a baculovirus expression vector system that is particularly tailored for the production of eukaryotic multiprotein complexes. Among other applications, MultiBac has been used to produce many important proteins and their complexes for their structural characterization, revealing fundamental cellular mechanisms.

Original languageEnglish
Pages (from-to)49-57
Number of pages9
JournalTrends in Biochemical Sciences
Volume37
Issue number2
DOIs
StatePublished - Feb 2012

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Multiprotein Complexes
Technology
Baculoviridae
Research
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

MultiBac : Expanding the research toolbox for multiprotein complexes. / Bieniossek, Christoph; Imasaki, Tsuyoshi; Takagi, Yuichiro; Berger, Imre.

In: Trends in Biochemical Sciences, Vol. 37, No. 2, 02.2012, p. 49-57.

Research output: Contribution to journalArticle

Bieniossek, Christoph ; Imasaki, Tsuyoshi ; Takagi, Yuichiro ; Berger, Imre. / MultiBac : Expanding the research toolbox for multiprotein complexes. In: Trends in Biochemical Sciences. 2012 ; Vol. 37, No. 2. pp. 49-57.
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