Multicenter comparison of lung and oral microbiomes of HIV-infected and HIV-uninfected individuals

Lung HIV Microbiome Project

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Rationale: Improved understanding of the lung microbiome in HIV-infected individuals could lead to better strategies for diagnosis, therapy, and prophylaxis of HIV-associated pneumonias. Differences in the oral and lung microbiomes in HIV-infected and HIV-uninfected individuals are not well defined. Whether highly active antiretroviral therapy influences these microbiomes is unclear. Objectives: Wedetermined whether oral and lungmicrobiomes differed in clinically healthy groups of HIV-infected and HIV-uninfected subjects. Methods: Participating sites in the Lung HIV Microbiome Project contributed bacterial 16S rRNA sequencing data from oral washes and bronchoalveolar lavages(BALs)obtainedfromHIV-uninfectedindividuals (n = 86),HIV-infected individuals whowere treatment naive (n = 18), and HIV-infected individuals receiving antiretroviral therapy (n = 38). Measurements and Main Results: Microbial populations differed in the oral washes among the subject groups (Streptococcus, Actinomyces, Rothia, and Atopobium), but there were no individual taxa that differed among the BALs. Comparison of oral washes and BALs demonstrated similar patterns from HIV-uninfected individuals and HIV-infected individuals receiving antiretroviral therapy, with multiple taxa differing in abundance. The pattern observed from HIV-infected individuals who were treatment naive differed from the other two groups, with differences limited to Veillonella, Rothia, and Granulicatella. CD4 cell counts did not influence the oral or BAL microbiome in these relatively healthy, HIV-infected subjects. Conclusions: The overall similarity of the microbiomes in participants with and without HIV infection was unexpected, because HIV-infected individuals with relatively preserved CD4 cell counts are at higher risk for lower respiratory tract infections, indicating impaired local immune function.

Original languageEnglish (US)
Pages (from-to)1335-1344
Number of pages10
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume192
Issue number11
DOIs
StatePublished - Dec 1 2015

Fingerprint

Microbiota
HIV
Lung
Bronchoalveolar Lavage
CD4 Lymphocyte Count
Veillonella
Therapeutics
Actinomyces
Highly Active Antiretroviral Therapy
Streptococcus
Respiratory Tract Infections
HIV Infections
Pneumonia

Keywords

  • Bronchoalveolar lavage
  • Bronchoscopy
  • HIV infection
  • Lung
  • Microbiome

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Multicenter comparison of lung and oral microbiomes of HIV-infected and HIV-uninfected individuals. / Lung HIV Microbiome Project.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 192, No. 11, 01.12.2015, p. 1335-1344.

Research output: Contribution to journalArticle

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abstract = "Rationale: Improved understanding of the lung microbiome in HIV-infected individuals could lead to better strategies for diagnosis, therapy, and prophylaxis of HIV-associated pneumonias. Differences in the oral and lung microbiomes in HIV-infected and HIV-uninfected individuals are not well defined. Whether highly active antiretroviral therapy influences these microbiomes is unclear. Objectives: Wedetermined whether oral and lungmicrobiomes differed in clinically healthy groups of HIV-infected and HIV-uninfected subjects. Methods: Participating sites in the Lung HIV Microbiome Project contributed bacterial 16S rRNA sequencing data from oral washes and bronchoalveolar lavages(BALs)obtainedfromHIV-uninfectedindividuals (n = 86),HIV-infected individuals whowere treatment naive (n = 18), and HIV-infected individuals receiving antiretroviral therapy (n = 38). Measurements and Main Results: Microbial populations differed in the oral washes among the subject groups (Streptococcus, Actinomyces, Rothia, and Atopobium), but there were no individual taxa that differed among the BALs. Comparison of oral washes and BALs demonstrated similar patterns from HIV-uninfected individuals and HIV-infected individuals receiving antiretroviral therapy, with multiple taxa differing in abundance. The pattern observed from HIV-infected individuals who were treatment naive differed from the other two groups, with differences limited to Veillonella, Rothia, and Granulicatella. CD4 cell counts did not influence the oral or BAL microbiome in these relatively healthy, HIV-infected subjects. Conclusions: The overall similarity of the microbiomes in participants with and without HIV infection was unexpected, because HIV-infected individuals with relatively preserved CD4 cell counts are at higher risk for lower respiratory tract infections, indicating impaired local immune function.",
keywords = "Bronchoalveolar lavage, Bronchoscopy, HIV infection, Lung, Microbiome",
author = "{Lung HIV Microbiome Project} and Beck, {James M.} and Schloss, {Patrick D.} and Arvind Venkataraman and Homer Twigg and Jablonski, {Kathleen A.} and Bushman, {Frederic D.} and Campbell, {Thomas B.} and Charlson, {Emily S.} and Collman, {Ronald G.} and Kristina Crothers and Curtis, {Jeffrey L.} and Drews, {Kimberly L.} and Flores, {Sonia C.} and Fontenot, {Andrew P.} and Foulkes, {Mary A.} and Ian Frank and Elodie Ghedin and Laurence Huang and Lynch, {Susan V.} and Alison Morris and Palmer, {Brent E.} and Schmidt, {Thomas M.} and Erica Sodergren and Weinstock, {George M.} and Young, {Vincent B.}",
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AU - Beck, James M.

AU - Schloss, Patrick D.

AU - Venkataraman, Arvind

AU - Twigg, Homer

AU - Jablonski, Kathleen A.

AU - Bushman, Frederic D.

AU - Campbell, Thomas B.

AU - Charlson, Emily S.

AU - Collman, Ronald G.

AU - Crothers, Kristina

AU - Curtis, Jeffrey L.

AU - Drews, Kimberly L.

AU - Flores, Sonia C.

AU - Fontenot, Andrew P.

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AU - Lynch, Susan V.

AU - Morris, Alison

AU - Palmer, Brent E.

AU - Schmidt, Thomas M.

AU - Sodergren, Erica

AU - Weinstock, George M.

AU - Young, Vincent B.

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N2 - Rationale: Improved understanding of the lung microbiome in HIV-infected individuals could lead to better strategies for diagnosis, therapy, and prophylaxis of HIV-associated pneumonias. Differences in the oral and lung microbiomes in HIV-infected and HIV-uninfected individuals are not well defined. Whether highly active antiretroviral therapy influences these microbiomes is unclear. Objectives: Wedetermined whether oral and lungmicrobiomes differed in clinically healthy groups of HIV-infected and HIV-uninfected subjects. Methods: Participating sites in the Lung HIV Microbiome Project contributed bacterial 16S rRNA sequencing data from oral washes and bronchoalveolar lavages(BALs)obtainedfromHIV-uninfectedindividuals (n = 86),HIV-infected individuals whowere treatment naive (n = 18), and HIV-infected individuals receiving antiretroviral therapy (n = 38). Measurements and Main Results: Microbial populations differed in the oral washes among the subject groups (Streptococcus, Actinomyces, Rothia, and Atopobium), but there were no individual taxa that differed among the BALs. Comparison of oral washes and BALs demonstrated similar patterns from HIV-uninfected individuals and HIV-infected individuals receiving antiretroviral therapy, with multiple taxa differing in abundance. The pattern observed from HIV-infected individuals who were treatment naive differed from the other two groups, with differences limited to Veillonella, Rothia, and Granulicatella. CD4 cell counts did not influence the oral or BAL microbiome in these relatively healthy, HIV-infected subjects. Conclusions: The overall similarity of the microbiomes in participants with and without HIV infection was unexpected, because HIV-infected individuals with relatively preserved CD4 cell counts are at higher risk for lower respiratory tract infections, indicating impaired local immune function.

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KW - Bronchoalveolar lavage

KW - Bronchoscopy

KW - HIV infection

KW - Lung

KW - Microbiome

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