Multicenter evaluation of Candida QuickFISH BC for identification of candida species directl'y from blood culture bottles

Ayman M. Abdelhamed, Sean X. Zhang, Tonya Watkins, Margie A. Morgan, Fann Wu, Rebecca J. Buckner, Deanna D. Fuller, Thomas Davis, Hossein Salimnia, Marilynn R. Fairfax, Paul R. Lephart, Melinda D. Poulter, Sarah B. Regi, Michael R. Jacobs

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Candida species are common causes of bloodstream infections (BSI), with high mortality. Four species cause>90% of Candida BSI: C. albicans, C. glabrata, C. parapsilosis, and C. tropicalis. Differentiation of Candida spp. is important because of differences in virulence and antimicrobial susceptibility. Candida QuickFISH BC, a multicolor, qualitative nucleic acid hybridization assay for the identification of C. albicans (green fluorescence), C. glabrata (red fluorescence), and C. parapsilosis (yellow fluorescence), was tested on Bactec and BacT/Alert blood culture bottles which signaled positive on automated blood culture devices and were positive for yeast by Gram stain at seven study sites. The results were compared to conventional identification. A total of 419 yeast-positive blood culture bottles were studied, consisting of 258 clinical samples (89 C. glabrata, 79 C. albicans, 23 C. parapsilosis, 18 C. tropicalis, and 49 other species) and 161 contrived samples inoculated with clinical isolates (40 C. glabrata, 46 C. albicans, 36 C. parapsilosis, 19 C. tropicalis, and 20 other species). A total of 415 samples contained a single fungal species, with C. glabrata (n = 129; 30.8%) being the most common isolate, followed by C. albicans (n = 125; 29.8%), C. parapsilosis (n = 59; 14.1%), C. tropicalis (n = 37; 8.8%), and C. krusei (n = 17; 4.1%). The overall agreement (with range for the three major Candida species) between the two methods was 99.3% (98.3 to 100%), with a sensitivity of 99.7% (98.3 to 100%) and a specificity of 98.0% (99.4 to 100%). This study showed that Candida QuickFISH BC is a rapid and accurate method for identifying C. albicans, C. glabrata, and C. parapsilosis, the three most common Candida species causing BSI, directly from blood culture bottles.

Original languageEnglish
Pages (from-to)1672-1676
Number of pages5
JournalJournal of Clinical Microbiology
Volume53
Issue number5
DOIs
StatePublished - May 1 2015

Fingerprint

Candida
Fluorescence
Yeasts
Infection
Nucleic Acid Hybridization
Blood Culture
Virulence
Equipment and Supplies
Mortality

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Abdelhamed, A. M., Zhang, S. X., Watkins, T., Morgan, M. A., Wu, F., Buckner, R. J., ... Jacobs, M. R. (2015). Multicenter evaluation of Candida QuickFISH BC for identification of candida species directl'y from blood culture bottles. Journal of Clinical Microbiology, 53(5), 1672-1676. https://doi.org/10.1128/JCM.00549-15

Multicenter evaluation of Candida QuickFISH BC for identification of candida species directl'y from blood culture bottles. / Abdelhamed, Ayman M.; Zhang, Sean X.; Watkins, Tonya; Morgan, Margie A.; Wu, Fann; Buckner, Rebecca J.; Fuller, Deanna D.; Davis, Thomas; Salimnia, Hossein; Fairfax, Marilynn R.; Lephart, Paul R.; Poulter, Melinda D.; Regi, Sarah B.; Jacobs, Michael R.

In: Journal of Clinical Microbiology, Vol. 53, No. 5, 01.05.2015, p. 1672-1676.

Research output: Contribution to journalArticle

Abdelhamed, AM, Zhang, SX, Watkins, T, Morgan, MA, Wu, F, Buckner, RJ, Fuller, DD, Davis, T, Salimnia, H, Fairfax, MR, Lephart, PR, Poulter, MD, Regi, SB & Jacobs, MR 2015, 'Multicenter evaluation of Candida QuickFISH BC for identification of candida species directl'y from blood culture bottles', Journal of Clinical Microbiology, vol. 53, no. 5, pp. 1672-1676. https://doi.org/10.1128/JCM.00549-15
Abdelhamed, Ayman M. ; Zhang, Sean X. ; Watkins, Tonya ; Morgan, Margie A. ; Wu, Fann ; Buckner, Rebecca J. ; Fuller, Deanna D. ; Davis, Thomas ; Salimnia, Hossein ; Fairfax, Marilynn R. ; Lephart, Paul R. ; Poulter, Melinda D. ; Regi, Sarah B. ; Jacobs, Michael R. / Multicenter evaluation of Candida QuickFISH BC for identification of candida species directl'y from blood culture bottles. In: Journal of Clinical Microbiology. 2015 ; Vol. 53, No. 5. pp. 1672-1676.
@article{31c31f23ab3b4df6ac49e2b2c943a719,
title = "Multicenter evaluation of Candida QuickFISH BC for identification of candida species directl'y from blood culture bottles",
abstract = "Candida species are common causes of bloodstream infections (BSI), with high mortality. Four species cause>90{\%} of Candida BSI: C. albicans, C. glabrata, C. parapsilosis, and C. tropicalis. Differentiation of Candida spp. is important because of differences in virulence and antimicrobial susceptibility. Candida QuickFISH BC, a multicolor, qualitative nucleic acid hybridization assay for the identification of C. albicans (green fluorescence), C. glabrata (red fluorescence), and C. parapsilosis (yellow fluorescence), was tested on Bactec and BacT/Alert blood culture bottles which signaled positive on automated blood culture devices and were positive for yeast by Gram stain at seven study sites. The results were compared to conventional identification. A total of 419 yeast-positive blood culture bottles were studied, consisting of 258 clinical samples (89 C. glabrata, 79 C. albicans, 23 C. parapsilosis, 18 C. tropicalis, and 49 other species) and 161 contrived samples inoculated with clinical isolates (40 C. glabrata, 46 C. albicans, 36 C. parapsilosis, 19 C. tropicalis, and 20 other species). A total of 415 samples contained a single fungal species, with C. glabrata (n = 129; 30.8{\%}) being the most common isolate, followed by C. albicans (n = 125; 29.8{\%}), C. parapsilosis (n = 59; 14.1{\%}), C. tropicalis (n = 37; 8.8{\%}), and C. krusei (n = 17; 4.1{\%}). The overall agreement (with range for the three major Candida species) between the two methods was 99.3{\%} (98.3 to 100{\%}), with a sensitivity of 99.7{\%} (98.3 to 100{\%}) and a specificity of 98.0{\%} (99.4 to 100{\%}). This study showed that Candida QuickFISH BC is a rapid and accurate method for identifying C. albicans, C. glabrata, and C. parapsilosis, the three most common Candida species causing BSI, directly from blood culture bottles.",
author = "Abdelhamed, {Ayman M.} and Zhang, {Sean X.} and Tonya Watkins and Morgan, {Margie A.} and Fann Wu and Buckner, {Rebecca J.} and Fuller, {Deanna D.} and Thomas Davis and Hossein Salimnia and Fairfax, {Marilynn R.} and Lephart, {Paul R.} and Poulter, {Melinda D.} and Regi, {Sarah B.} and Jacobs, {Michael R.}",
year = "2015",
month = "5",
day = "1",
doi = "10.1128/JCM.00549-15",
language = "English",
volume = "53",
pages = "1672--1676",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "5",

}

TY - JOUR

T1 - Multicenter evaluation of Candida QuickFISH BC for identification of candida species directl'y from blood culture bottles

AU - Abdelhamed, Ayman M.

AU - Zhang, Sean X.

AU - Watkins, Tonya

AU - Morgan, Margie A.

AU - Wu, Fann

AU - Buckner, Rebecca J.

AU - Fuller, Deanna D.

AU - Davis, Thomas

AU - Salimnia, Hossein

AU - Fairfax, Marilynn R.

AU - Lephart, Paul R.

AU - Poulter, Melinda D.

AU - Regi, Sarah B.

AU - Jacobs, Michael R.

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Candida species are common causes of bloodstream infections (BSI), with high mortality. Four species cause>90% of Candida BSI: C. albicans, C. glabrata, C. parapsilosis, and C. tropicalis. Differentiation of Candida spp. is important because of differences in virulence and antimicrobial susceptibility. Candida QuickFISH BC, a multicolor, qualitative nucleic acid hybridization assay for the identification of C. albicans (green fluorescence), C. glabrata (red fluorescence), and C. parapsilosis (yellow fluorescence), was tested on Bactec and BacT/Alert blood culture bottles which signaled positive on automated blood culture devices and were positive for yeast by Gram stain at seven study sites. The results were compared to conventional identification. A total of 419 yeast-positive blood culture bottles were studied, consisting of 258 clinical samples (89 C. glabrata, 79 C. albicans, 23 C. parapsilosis, 18 C. tropicalis, and 49 other species) and 161 contrived samples inoculated with clinical isolates (40 C. glabrata, 46 C. albicans, 36 C. parapsilosis, 19 C. tropicalis, and 20 other species). A total of 415 samples contained a single fungal species, with C. glabrata (n = 129; 30.8%) being the most common isolate, followed by C. albicans (n = 125; 29.8%), C. parapsilosis (n = 59; 14.1%), C. tropicalis (n = 37; 8.8%), and C. krusei (n = 17; 4.1%). The overall agreement (with range for the three major Candida species) between the two methods was 99.3% (98.3 to 100%), with a sensitivity of 99.7% (98.3 to 100%) and a specificity of 98.0% (99.4 to 100%). This study showed that Candida QuickFISH BC is a rapid and accurate method for identifying C. albicans, C. glabrata, and C. parapsilosis, the three most common Candida species causing BSI, directly from blood culture bottles.

AB - Candida species are common causes of bloodstream infections (BSI), with high mortality. Four species cause>90% of Candida BSI: C. albicans, C. glabrata, C. parapsilosis, and C. tropicalis. Differentiation of Candida spp. is important because of differences in virulence and antimicrobial susceptibility. Candida QuickFISH BC, a multicolor, qualitative nucleic acid hybridization assay for the identification of C. albicans (green fluorescence), C. glabrata (red fluorescence), and C. parapsilosis (yellow fluorescence), was tested on Bactec and BacT/Alert blood culture bottles which signaled positive on automated blood culture devices and were positive for yeast by Gram stain at seven study sites. The results were compared to conventional identification. A total of 419 yeast-positive blood culture bottles were studied, consisting of 258 clinical samples (89 C. glabrata, 79 C. albicans, 23 C. parapsilosis, 18 C. tropicalis, and 49 other species) and 161 contrived samples inoculated with clinical isolates (40 C. glabrata, 46 C. albicans, 36 C. parapsilosis, 19 C. tropicalis, and 20 other species). A total of 415 samples contained a single fungal species, with C. glabrata (n = 129; 30.8%) being the most common isolate, followed by C. albicans (n = 125; 29.8%), C. parapsilosis (n = 59; 14.1%), C. tropicalis (n = 37; 8.8%), and C. krusei (n = 17; 4.1%). The overall agreement (with range for the three major Candida species) between the two methods was 99.3% (98.3 to 100%), with a sensitivity of 99.7% (98.3 to 100%) and a specificity of 98.0% (99.4 to 100%). This study showed that Candida QuickFISH BC is a rapid and accurate method for identifying C. albicans, C. glabrata, and C. parapsilosis, the three most common Candida species causing BSI, directly from blood culture bottles.

UR - http://www.scopus.com/inward/record.url?scp=84928007428&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84928007428&partnerID=8YFLogxK

U2 - 10.1128/JCM.00549-15

DO - 10.1128/JCM.00549-15

M3 - Article

C2 - 25762766

AN - SCOPUS:84928007428

VL - 53

SP - 1672

EP - 1676

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 5

ER -