Multicenter evaluation of the portrait staph ID/R blood culture panel for rapid identification of staphylococci and detection of the mecA gene

Gerald Denys, Vanessa Collazo-Velez, Stephen Young, Judy A. Daly, Marc Roger Couturier, Matthew L. Faron, Blake W. Buchan, Nathan Ledeboer

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Bloodstream infections are a leading cause of morbidity and mortality in the United States and are associated with increased health care costs. We evaluated the Portrait Staph ID/R blood culture panel (BCP) multiplex PCR assay (Great Basin Scientific, Salt Lake City, UT) for the rapid and simultaneous identification (ID) of Staphylococcus aureus, Staphylococcus lugdunensis, and Staphylococcus species to the genus level and the detection of the mecA gene directly from a positive blood culture bottle. A total of 765 Bactec bottles demonstrating Gram-positive cocci in singles or clusters were tested during the prospective trial at 3 clinical sites. The Portrait Staph ID/R BCP results were compared with results from conventional biochemical and cefoxitin disk methods performed at an independent laboratory. Discordant ID and mecA results were resolved by rpoB gene sequencing and mecA gene sequencing, respectively. A total of 658 Staphylococcus species isolates (S. aureus, 211 isolates; S. lugdunensis, 3 isolates; and Staphylococcus spp., 444 isolates) were recovered from monomicrobial and 33 polymicrobial blood cultures. After discrepant analysis, the overall ratios of Portrait Staph ID/R BCP positive percent agreement and negative percent agreement were 99.4%/99.9% for Staphylococcus ID and 99.7%/99.2% for mecA detection.

Original languageEnglish (US)
Pages (from-to)1140-1146
Number of pages7
JournalJournal of clinical microbiology
Volume55
Issue number4
DOIs
StatePublished - Apr 1 2017

Keywords

  • Blood culture
  • Genotypic identification
  • MecA detection
  • PCR
  • Rapid tests
  • Staphylococcus

ASJC Scopus subject areas

  • Microbiology (medical)

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    Denys, G., Collazo-Velez, V., Young, S., Daly, J. A., Couturier, M. R., Faron, M. L., Buchan, B. W., & Ledeboer, N. (2017). Multicenter evaluation of the portrait staph ID/R blood culture panel for rapid identification of staphylococci and detection of the mecA gene. Journal of clinical microbiology, 55(4), 1140-1146. https://doi.org/10.1128/JCM.02348-16