Multiphoton imaging techniques in acute kidney injury

Bruce Molitoris, Ruben M. Sandoval

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Citations (Scopus)

Abstract

Multiphoton microscopy allows investigators the opportunity to study dynamic events within the functioning kidney and during acute kidney injury. This enables investigators to follow complex multifactorial processes in the kidney with improved spatial and temporal resolution, and sensitivity. Furthermore, the ability to obtain volumetric data (3-D) makes quantitative 4-D (time) analysis possible. Finally, use of up to three fluorophores concurrently in multiphoton microscopy allows for three different or interactive processes to be observed simultaneously. Therefore, this approach compliments existing molecular, biochemical and pharmacologic techniques by allowing for direct visualization at the cellular and subcellular levels for molecules without the requirement for fixation. Its use in acute kidney injury is in its infancy but offers much promise for unraveling the complex interdependent processes known to contribute to cell injury and organ failure.

Original languageEnglish
Title of host publicationContributions to Nephrology
Pages46-53
Number of pages8
Volume165
DOIs
StatePublished - Apr 2010

Publication series

NameContributions to Nephrology
Volume165
ISSN (Print)03025144

Fingerprint

Acute Kidney Injury
Microscopy
Research Personnel
Kidney
Wounds and Injuries

ASJC Scopus subject areas

  • Nephrology

Cite this

Molitoris, B., & Sandoval, R. M. (2010). Multiphoton imaging techniques in acute kidney injury. In Contributions to Nephrology (Vol. 165, pp. 46-53). (Contributions to Nephrology; Vol. 165). https://doi.org/10.1159/000313744

Multiphoton imaging techniques in acute kidney injury. / Molitoris, Bruce; Sandoval, Ruben M.

Contributions to Nephrology. Vol. 165 2010. p. 46-53 (Contributions to Nephrology; Vol. 165).

Research output: Chapter in Book/Report/Conference proceedingChapter

Molitoris, B & Sandoval, RM 2010, Multiphoton imaging techniques in acute kidney injury. in Contributions to Nephrology. vol. 165, Contributions to Nephrology, vol. 165, pp. 46-53. https://doi.org/10.1159/000313744
Molitoris B, Sandoval RM. Multiphoton imaging techniques in acute kidney injury. In Contributions to Nephrology. Vol. 165. 2010. p. 46-53. (Contributions to Nephrology). https://doi.org/10.1159/000313744
Molitoris, Bruce ; Sandoval, Ruben M. / Multiphoton imaging techniques in acute kidney injury. Contributions to Nephrology. Vol. 165 2010. pp. 46-53 (Contributions to Nephrology).
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