Multiphoton imaging techniques in acute kidney injury

Bruce A. Molitoris, Ruben M. Sandoval

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Scopus citations

Abstract

Multiphoton microscopy allows investigators the opportunity to study dynamic events within the functioning kidney and during acute kidney injury. This enables investigators to follow complex multifactorial processes in the kidney with improved spatial and temporal resolution, and sensitivity. Furthermore, the ability to obtain volumetric data (3-D) makes quantitative 4-D (time) analysis possible. Finally, use of up to three fluorophores concurrently in multiphoton microscopy allows for three different or interactive processes to be observed simultaneously. Therefore, this approach compliments existing molecular, biochemical and pharmacologic techniques by allowing for direct visualization at the cellular and subcellular levels for molecules without the requirement for fixation. Its use in acute kidney injury is in its infancy but offers much promise for unraveling the complex interdependent processes known to contribute to cell injury and organ failure.

Original languageEnglish (US)
Title of host publicationCardiorenal Syndromes in Critical Care
EditorsClaudio Ronco, Rinaldo Bellomo, Peter McCullough
Pages46-53
Number of pages8
DOIs
StatePublished - Apr 1 2010

Publication series

NameContributions to Nephrology
Volume165
ISSN (Print)0302-5144

ASJC Scopus subject areas

  • Nephrology

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    Molitoris, B. A., & Sandoval, R. M. (2010). Multiphoton imaging techniques in acute kidney injury. In C. Ronco, R. Bellomo, & P. McCullough (Eds.), Cardiorenal Syndromes in Critical Care (pp. 46-53). (Contributions to Nephrology; Vol. 165). https://doi.org/10.1159/000313744