Multiple Class I and Class II Haemophilus ducreyi Strains Cause Cutaneous Ulcers in Children on an Endemic Island

Jacob C. Grant, Camila González-Beiras, Kristen M. Amick, Kate R. Fortney, Dharanesh Gangaiah, Tricia L. Humphreys, Oriol Mitjà, Ana Abecasis, Stanley Spinola

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Together with Treponema pallidum subspecies pertenue, Haemophilus ducreyi is a major cause of exudative cutaneous ulcers (CUs) in children. For H. ducreyi, both class I and class II strains, asymptomatic colonization, and environmental reservoirs have been found in endemic regions, but the epidemiology of this infection is unknown. Methods: Based on published whole-genome sequences of H. ducreyi CU strains, a single-locus typing system was developed and applied to H. ducreyi-positive CU samples obtained prior to, 1 year after, and 2 years after the initiation of a mass drug administration campaign to eradicate CU on Lihir Island in Papua New Guinea. DNA from the CU samples was amplified with class I and class II dsrA-specific primers and sequenced; the samples were classified into dsrA types, which were geospatially mapped. Selection pressure analysis was performed on the dsrA sequences. Results: Thirty-seven samples contained class I sequences, 27 contained class II sequences, and 13 contained both. There were 5 class I and 4 class II types circulating on the island; 3 types accounted for approximately 87% of the strains. The composition and geospatial distribution of the types varied little over time and there was no evidence of selection pressure. Conclusions: Multiple strains of H. ducreyi cause CU on an endemic island and coinfections are common. In contrast to recent findings with T. pallidum pertenue, strain composition is not affected by antibiotic pressure, consistent with environmental reservoirs of H. ducreyi. Such reservoirs must be addressed to achieve eradication of H. ducreyi.

Original languageEnglish (US)
Pages (from-to)1768-1774
Number of pages7
JournalClinical infectious diseases : an official publication of the Infectious Diseases Society of America
Volume67
Issue number11
DOIs
StatePublished - Nov 13 2018

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Haemophilus ducreyi
Skin Ulcer
Islands
Treponema pallidum
Pressure
Papua New Guinea
Coinfection
Epidemiology
Genome
Anti-Bacterial Agents
DNA

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Multiple Class I and Class II Haemophilus ducreyi Strains Cause Cutaneous Ulcers in Children on an Endemic Island. / Grant, Jacob C.; González-Beiras, Camila; Amick, Kristen M.; Fortney, Kate R.; Gangaiah, Dharanesh; Humphreys, Tricia L.; Mitjà, Oriol; Abecasis, Ana; Spinola, Stanley.

In: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, Vol. 67, No. 11, 13.11.2018, p. 1768-1774.

Research output: Contribution to journalArticle

Grant, Jacob C. ; González-Beiras, Camila ; Amick, Kristen M. ; Fortney, Kate R. ; Gangaiah, Dharanesh ; Humphreys, Tricia L. ; Mitjà, Oriol ; Abecasis, Ana ; Spinola, Stanley. / Multiple Class I and Class II Haemophilus ducreyi Strains Cause Cutaneous Ulcers in Children on an Endemic Island. In: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 2018 ; Vol. 67, No. 11. pp. 1768-1774.
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AU - Grant, Jacob C.

AU - González-Beiras, Camila

AU - Amick, Kristen M.

AU - Fortney, Kate R.

AU - Gangaiah, Dharanesh

AU - Humphreys, Tricia L.

AU - Mitjà, Oriol

AU - Abecasis, Ana

AU - Spinola, Stanley

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N2 - Background: Together with Treponema pallidum subspecies pertenue, Haemophilus ducreyi is a major cause of exudative cutaneous ulcers (CUs) in children. For H. ducreyi, both class I and class II strains, asymptomatic colonization, and environmental reservoirs have been found in endemic regions, but the epidemiology of this infection is unknown. Methods: Based on published whole-genome sequences of H. ducreyi CU strains, a single-locus typing system was developed and applied to H. ducreyi-positive CU samples obtained prior to, 1 year after, and 2 years after the initiation of a mass drug administration campaign to eradicate CU on Lihir Island in Papua New Guinea. DNA from the CU samples was amplified with class I and class II dsrA-specific primers and sequenced; the samples were classified into dsrA types, which were geospatially mapped. Selection pressure analysis was performed on the dsrA sequences. Results: Thirty-seven samples contained class I sequences, 27 contained class II sequences, and 13 contained both. There were 5 class I and 4 class II types circulating on the island; 3 types accounted for approximately 87% of the strains. The composition and geospatial distribution of the types varied little over time and there was no evidence of selection pressure. Conclusions: Multiple strains of H. ducreyi cause CU on an endemic island and coinfections are common. In contrast to recent findings with T. pallidum pertenue, strain composition is not affected by antibiotic pressure, consistent with environmental reservoirs of H. ducreyi. Such reservoirs must be addressed to achieve eradication of H. ducreyi.

AB - Background: Together with Treponema pallidum subspecies pertenue, Haemophilus ducreyi is a major cause of exudative cutaneous ulcers (CUs) in children. For H. ducreyi, both class I and class II strains, asymptomatic colonization, and environmental reservoirs have been found in endemic regions, but the epidemiology of this infection is unknown. Methods: Based on published whole-genome sequences of H. ducreyi CU strains, a single-locus typing system was developed and applied to H. ducreyi-positive CU samples obtained prior to, 1 year after, and 2 years after the initiation of a mass drug administration campaign to eradicate CU on Lihir Island in Papua New Guinea. DNA from the CU samples was amplified with class I and class II dsrA-specific primers and sequenced; the samples were classified into dsrA types, which were geospatially mapped. Selection pressure analysis was performed on the dsrA sequences. Results: Thirty-seven samples contained class I sequences, 27 contained class II sequences, and 13 contained both. There were 5 class I and 4 class II types circulating on the island; 3 types accounted for approximately 87% of the strains. The composition and geospatial distribution of the types varied little over time and there was no evidence of selection pressure. Conclusions: Multiple strains of H. ducreyi cause CU on an endemic island and coinfections are common. In contrast to recent findings with T. pallidum pertenue, strain composition is not affected by antibiotic pressure, consistent with environmental reservoirs of H. ducreyi. Such reservoirs must be addressed to achieve eradication of H. ducreyi.

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