Multiple functions of human single-stranded-DNA binding protein in simian virus 40 DNA replication: Single-strand stabilization and stimulation of DNA polymerases α and δ

M. K. Kenny, S. H. Lee, J. Hurwitz

Research output: Contribution to journalArticle

158 Scopus citations

Abstract

The human single-stranded-DNA binding protein (human SSB) is required for simian virus 40 (SV40) DNA replication in vitro. SV40 larger tumor antigen and human SSB can support extensive unwinding of SV40 origin-containing DNA in the presence of ATP and a topoisomerase that relieves positive superhelicity. Although SSBs from viral and prokaryotic sources substituted for human SSB in the DNA-unwinding reaction, they did not substitute in the replication of SV40 DNA. The specificity for human SSB in SV40 DNA replication can be explained, at least in part, by the finding that DNA polymerase α was stimulated 10-fold by human SSB but not by other SSBs. Human SSB also stimulated proliferating-cell nuclear antigen-dependent DNA polymerase δ; however, other SSBs stimulated this polymerase as well.

Original languageEnglish (US)
Pages (from-to)9757-9761
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number24
DOIs
StatePublished - Dec 1 1989

Keywords

  • DNA unwinding
  • proliferating-cell nuclear antigen
  • simian virus 40 large tumor antigen

ASJC Scopus subject areas

  • General

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