Multiple lymphokine production by a phorbol ester-stimulated mouse thymoma

Relationship to cell cycle events

K. T. Pearlstein, L. Staiano-Coico, R. A. Miller, Louis Pelus, M. E. Kirch, O. Stutman, M. A. Palladino

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Interleukin 2 (IL-2) production was studied in a subclone of the murine thymoma EL 4. Phenotypic characterization revealed the EL 4-17-2 line to be Thy-1.2+, Lyt-1.2+, and Lyt-2.2-. Costimulation with 500 ng 12-O-tetradecanoylphorbol 13-acetate (TPA)/ml and 5 μg concanavalin A (Con A)/ml induced optimal levels of IL-2. Three related phorbol esters stimulated comparable levels of IL-2 when used in conjunction with Con A. Kinetic experiments indicated that IL-2 first became detectable at 2 hours in TPA-treated cultures, whereas in cultures stimulated with Con A alone IL-2 production was not evident until 8 hours. Flow cytometry indicated that TPA and its related phorbol esters cause a perturbation in the cycling of the cell which may be related to increased IL-2 production. Under the conditions examined, no interferon-γ (IFN-γ) was detectable. Conversely, both granulocyte-macrophage colony-stimulating factor (CSF-GM) and interleukin-3 (IL-3) were found under conditions that led to stimulation of IL-2 synthesis. CSF-GM was produced in cultures treated singly with 500 ng TPA/ml or with Con A. IL-3 production was similar to IL-2 production, because optimal levels were found in cultures after combined treatment with phorbol ester and mitogen.

Original languageEnglish (US)
Pages (from-to)583-590
Number of pages8
JournalJournal of the National Cancer Institute
Volume71
Issue number3
StatePublished - 1983
Externally publishedYes

Fingerprint

Thymoma
Lymphokines
Phorbol Esters
Interleukin-2
Cell Cycle
Tetradecanoylphorbol Acetate
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-3
Concanavalin A
Mitogens
Interferons
Flow Cytometry

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Pearlstein, K. T., Staiano-Coico, L., Miller, R. A., Pelus, L., Kirch, M. E., Stutman, O., & Palladino, M. A. (1983). Multiple lymphokine production by a phorbol ester-stimulated mouse thymoma: Relationship to cell cycle events. Journal of the National Cancer Institute, 71(3), 583-590.

Multiple lymphokine production by a phorbol ester-stimulated mouse thymoma : Relationship to cell cycle events. / Pearlstein, K. T.; Staiano-Coico, L.; Miller, R. A.; Pelus, Louis; Kirch, M. E.; Stutman, O.; Palladino, M. A.

In: Journal of the National Cancer Institute, Vol. 71, No. 3, 1983, p. 583-590.

Research output: Contribution to journalArticle

Pearlstein, KT, Staiano-Coico, L, Miller, RA, Pelus, L, Kirch, ME, Stutman, O & Palladino, MA 1983, 'Multiple lymphokine production by a phorbol ester-stimulated mouse thymoma: Relationship to cell cycle events', Journal of the National Cancer Institute, vol. 71, no. 3, pp. 583-590.
Pearlstein KT, Staiano-Coico L, Miller RA, Pelus L, Kirch ME, Stutman O et al. Multiple lymphokine production by a phorbol ester-stimulated mouse thymoma: Relationship to cell cycle events. Journal of the National Cancer Institute. 1983;71(3):583-590.
Pearlstein, K. T. ; Staiano-Coico, L. ; Miller, R. A. ; Pelus, Louis ; Kirch, M. E. ; Stutman, O. ; Palladino, M. A. / Multiple lymphokine production by a phorbol ester-stimulated mouse thymoma : Relationship to cell cycle events. In: Journal of the National Cancer Institute. 1983 ; Vol. 71, No. 3. pp. 583-590.
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