Multiple lymphokine production by a phorbol ester-stimulated mouse thymoma: Relationship to cell cycle events

K. T. Pearlstein, L. Staiano-Coico, R. A. Miller, L. M. Pelus, M. E. Kirch, O. Stutman, M. A. Palladino

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Interleukin 2 (IL-2) production was studied in a subclone of the murine thymoma EL 4. Phenotypic characterization revealed the EL 4-17-2 line to be Thy-1.2+, Lyt-1.2+, and Lyt-2.2-. Costimulation with 500 ng 12-O-tetradecanoylphorbol 13-acetate (TPA)/ml and 5 μg concanavalin A (Con A)/ml induced optimal levels of IL-2. Three related phorbol esters stimulated comparable levels of IL-2 when used in conjunction with Con A. Kinetic experiments indicated that IL-2 first became detectable at 2 hours in TPA-treated cultures, whereas in cultures stimulated with Con A alone IL-2 production was not evident until 8 hours. Flow cytometry indicated that TPA and its related phorbol esters cause a perturbation in the cycling of the cell which may be related to increased IL-2 production. Under the conditions examined, no interferon-γ (IFN-γ) was detectable. Conversely, both granulocyte-macrophage colony-stimulating factor (CSF-GM) and interleukin-3 (IL-3) were found under conditions that led to stimulation of IL-2 synthesis. CSF-GM was produced in cultures treated singly with 500 ng TPA/ml or with Con A. IL-3 production was similar to IL-2 production, because optimal levels were found in cultures after combined treatment with phorbol ester and mitogen.

Original languageEnglish (US)
Pages (from-to)583-590
Number of pages8
JournalJournal of the National Cancer Institute
Volume71
Issue number3
StatePublished - Dec 8 1983

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Pearlstein, K. T., Staiano-Coico, L., Miller, R. A., Pelus, L. M., Kirch, M. E., Stutman, O., & Palladino, M. A. (1983). Multiple lymphokine production by a phorbol ester-stimulated mouse thymoma: Relationship to cell cycle events. Journal of the National Cancer Institute, 71(3), 583-590.