Abstract
Aging of the human retina is characterized by progressive pathology, which can lead to vision loss. This progression is believed to involve reactive metabolic intermediates reacting with constituents of Bruch's membrane, significantly altering its physiochemical nature and function. We aimed to replace a myriad of techniques following these changes with one, Raman spectroscopy. We used multiplexed Raman spectroscopy to analyze the age-related changes in 7 proteins, 3 lipids, and 8 advanced glycation/lipoxidation endproducts (AGEs/ALEs) in 63 postmortem human donors. We provided an important database for Raman spectra from a broad range of AGEs and ALEs, each with a characteristic fingerprint. Many of these adducts were shown for the first time in human Bruch's membrane and are significantly associated with aging. The study also introduced the previously unreported up-regulation of heme during aging of Bruch's membrane, which is associated with AGE/ALE formation. Selection of donors ranged from ages 32 to 92 yr. We demonstrated that Raman spectroscopy can identify and quantify age-related changes in a single nondestructive measurement, with potential to measure age-related changes in vivo. We present the first directly recorded evidence of the key role of heme in AGE/ALE formation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4816-4824 |
| Number of pages | 9 |
| Journal | FASEB Journal |
| Volume | 24 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 2010 |
| Externally published | Yes |
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Keywords
- Advanced glycation endproducts
- Advanced lipoxidation endproducts
- Aging
- Hemoglobin
- Raman spectroscopy
ASJC Scopus subject areas
- Biochemistry
- Biotechnology
- Genetics
- Molecular Biology
Cite this
Multiplex analysis of age-related protein and lipid modifications in human Bruch's membrane. / Beattie, J. Renwick; Pawlak, Anna M.; Boulton, Michael E.; Zhang, Jianye; Monnier, Vincent M.; McGarvey, John J.; Stitt, Alan W.
In: FASEB Journal, Vol. 24, No. 12, 12.2010, p. 4816-4824.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Multiplex analysis of age-related protein and lipid modifications in human Bruch's membrane
AU - Beattie, J. Renwick
AU - Pawlak, Anna M.
AU - Boulton, Michael E.
AU - Zhang, Jianye
AU - Monnier, Vincent M.
AU - McGarvey, John J.
AU - Stitt, Alan W.
PY - 2010/12
Y1 - 2010/12
N2 - Aging of the human retina is characterized by progressive pathology, which can lead to vision loss. This progression is believed to involve reactive metabolic intermediates reacting with constituents of Bruch's membrane, significantly altering its physiochemical nature and function. We aimed to replace a myriad of techniques following these changes with one, Raman spectroscopy. We used multiplexed Raman spectroscopy to analyze the age-related changes in 7 proteins, 3 lipids, and 8 advanced glycation/lipoxidation endproducts (AGEs/ALEs) in 63 postmortem human donors. We provided an important database for Raman spectra from a broad range of AGEs and ALEs, each with a characteristic fingerprint. Many of these adducts were shown for the first time in human Bruch's membrane and are significantly associated with aging. The study also introduced the previously unreported up-regulation of heme during aging of Bruch's membrane, which is associated with AGE/ALE formation. Selection of donors ranged from ages 32 to 92 yr. We demonstrated that Raman spectroscopy can identify and quantify age-related changes in a single nondestructive measurement, with potential to measure age-related changes in vivo. We present the first directly recorded evidence of the key role of heme in AGE/ALE formation.
AB - Aging of the human retina is characterized by progressive pathology, which can lead to vision loss. This progression is believed to involve reactive metabolic intermediates reacting with constituents of Bruch's membrane, significantly altering its physiochemical nature and function. We aimed to replace a myriad of techniques following these changes with one, Raman spectroscopy. We used multiplexed Raman spectroscopy to analyze the age-related changes in 7 proteins, 3 lipids, and 8 advanced glycation/lipoxidation endproducts (AGEs/ALEs) in 63 postmortem human donors. We provided an important database for Raman spectra from a broad range of AGEs and ALEs, each with a characteristic fingerprint. Many of these adducts were shown for the first time in human Bruch's membrane and are significantly associated with aging. The study also introduced the previously unreported up-regulation of heme during aging of Bruch's membrane, which is associated with AGE/ALE formation. Selection of donors ranged from ages 32 to 92 yr. We demonstrated that Raman spectroscopy can identify and quantify age-related changes in a single nondestructive measurement, with potential to measure age-related changes in vivo. We present the first directly recorded evidence of the key role of heme in AGE/ALE formation.
KW - Advanced glycation endproducts
KW - Advanced lipoxidation endproducts
KW - Aging
KW - Hemoglobin
KW - Raman spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=78649730161&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78649730161&partnerID=8YFLogxK
U2 - 10.1096/fj.10-166090
DO - 10.1096/fj.10-166090
M3 - Article
C2 - 20686107
AN - SCOPUS:78649730161
VL - 24
SP - 4816
EP - 4824
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 12
ER -