Multiplex PCR amplification of three microsatellites within the CFTR gene

Núria Morral, Xavier Estivill

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Multiplex PCR amplification has been developed for three highly polymorphic microsatellites (IVS8CA, IVS17BTA, and IVS17BCA) located in intronic regions of the CFTR (cystic fibrosis (CF) transmembrane conductance regulator) gene. The triplex PCR reaction required different concentrations of each pair of primers and labeling of primers in the same reaction. Total informativity is obtained in 90.25% of couples requiring analysis of polymorphisms, and when triplex microsatellite analysis is combined with analysis for the six most common CF mutations in the Spanish population, informativity reaches more than 99%.

Original languageEnglish (US)
Pages (from-to)1362-1364
Number of pages3
JournalGenomics
Volume13
Issue number4
DOIs
StatePublished - Aug 1992
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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