Multiscale analysis of morphology and mechanics in tail tendon from the ZDSD rat model of type 2 diabetes

Armando Diaz Gonzalez, Maxime A. Gallant, David Burr, Joseph M. Wallace

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Type 2 diabetes (T2D) impacts multiple organ systems including the circulatory, renal, nervous and musculoskeletal systems. In collagen-based tissues, one mechanism that may be responsible for detrimental mechanical impacts of T2D is the formation of advanced glycation end products (AGEs) leading to increased collagen stiffness and decreased toughness, resulting in brittle tissue behavior. The purpose of this study was to investigate tendon mechanical properties from normal and diabetic rats at two distinct length scales, testing the hypothesis that increased stiffness and strength and decreased toughness at the fiber level would be associated with alterations in nanoscale morphology and mechanics. Individual fascicles from female Zucker diabetic Sprague-Dawley (ZDSD) rats had no differences in fascicle-level mechanical properties but had increased material-level strength and stiffness versus control rats (CD). At the nanoscale, collagen fibril D-spacing was shifted towards higher spacing values in diabetic ZDSD fibrils. The distribution of nanoscale modulus values was also shifted to higher values. Material-level strength and stiffness from whole fiber tests were increased in ZDSD tails. Correlations between nanoscale and microscale properties indicate a direct positive relationship between the two length scales, most notably in the relationship between nanoscale and microscale modulus. These findings indicate that diabetes-induced changes in material strength and modulus were driven by alterations at the nanoscale.

Original languageEnglish
Pages (from-to)681-686
Number of pages6
JournalJournal of Biomechanics
Volume47
Issue number3
DOIs
StatePublished - Feb 7 2014

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Tendons
Medical problems
Mechanics
Type 2 Diabetes Mellitus
Sprague Dawley Rats
Tail
Rats
Collagen
Stiffness
Toughness
Musculoskeletal System
Advanced Glycosylation End Products
Rat control
Musculoskeletal system
Tissue
Cardiovascular System
Mechanical properties
Nervous System
Fibers
Neurology

Keywords

  • AFM
  • Modulus
  • Nanoscale
  • Tension
  • Ultrastructure

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Rehabilitation
  • Biophysics
  • Biomedical Engineering

Cite this

Multiscale analysis of morphology and mechanics in tail tendon from the ZDSD rat model of type 2 diabetes. / Gonzalez, Armando Diaz; Gallant, Maxime A.; Burr, David; Wallace, Joseph M.

In: Journal of Biomechanics, Vol. 47, No. 3, 07.02.2014, p. 681-686.

Research output: Contribution to journalArticle

Gonzalez, Armando Diaz ; Gallant, Maxime A. ; Burr, David ; Wallace, Joseph M. / Multiscale analysis of morphology and mechanics in tail tendon from the ZDSD rat model of type 2 diabetes. In: Journal of Biomechanics. 2014 ; Vol. 47, No. 3. pp. 681-686.
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