Munc18c is not rate-limiting for glucose and long-chain fatty acid uptake in the heart

Daphna D J Habets, Debbie C. Thurmond, Will A. Coumans, Arend Bonen, Jan F C Glatz, Joost J F P Luiken

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The role of soluble N -ethylmaleimide-sensitive factor attachment protein receptor (SNARE)- and SNARE-associated proteins have not yet been assessed in regulation of cardiac glucose uptake, nor in the regulation of long-chain fatty acid (LCFA) uptake in any tissue. Munc18c is a SNARE-associated protein that regulates GLUT4 translocation in skeletal muscle and adipose tissue. Using cardiomyocytes from Munc18c-/+ mice (with 56% reduction of Munc18c protein expression), we investigated whether this syntaxin4-associated protein is involved in regulation of cardiac substrate uptake. Basal, insulin- and oligomycin (a 5′ AMP-activated protein kinase-activating agent)-stimulated glucose and LCFA uptake were not altered significantly in Munc18c-/+ cardiomyocytes compared to wild-type cells. We conclude, therefore, that Munc18c is not rate-limiting for cardiac substrate uptake, neither under basal conditions nor when maximally stimulated metabolically.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalMolecular and Cellular Biochemistry
Volume322
Issue number1-2
DOIs
StatePublished - 2009

Fingerprint

Fatty Acids
Glucose
Cardiac Myocytes
Munc18 Proteins
Proteins
Glucose Transporter Type 4
Oligomycins
SNARE Proteins
AMP-Activated Protein Kinases
Tissue
Adipose Tissue
Substrates
Skeletal Muscle
Muscle
Insulin
Muscles

Keywords

  • Cardiomyocytes
  • Glucose uptake
  • Long-chain fatty acid uptake
  • Munc18c

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Habets, D. D. J., Thurmond, D. C., Coumans, W. A., Bonen, A., Glatz, J. F. C., & Luiken, J. J. F. P. (2009). Munc18c is not rate-limiting for glucose and long-chain fatty acid uptake in the heart. Molecular and Cellular Biochemistry, 322(1-2), 81-86. https://doi.org/10.1007/s11010-008-9942-y

Munc18c is not rate-limiting for glucose and long-chain fatty acid uptake in the heart. / Habets, Daphna D J; Thurmond, Debbie C.; Coumans, Will A.; Bonen, Arend; Glatz, Jan F C; Luiken, Joost J F P.

In: Molecular and Cellular Biochemistry, Vol. 322, No. 1-2, 2009, p. 81-86.

Research output: Contribution to journalArticle

Habets, DDJ, Thurmond, DC, Coumans, WA, Bonen, A, Glatz, JFC & Luiken, JJFP 2009, 'Munc18c is not rate-limiting for glucose and long-chain fatty acid uptake in the heart', Molecular and Cellular Biochemistry, vol. 322, no. 1-2, pp. 81-86. https://doi.org/10.1007/s11010-008-9942-y
Habets, Daphna D J ; Thurmond, Debbie C. ; Coumans, Will A. ; Bonen, Arend ; Glatz, Jan F C ; Luiken, Joost J F P. / Munc18c is not rate-limiting for glucose and long-chain fatty acid uptake in the heart. In: Molecular and Cellular Biochemistry. 2009 ; Vol. 322, No. 1-2. pp. 81-86.
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