Munc18c phosphorylation by the insulin receptor links cell signaling directly to SNARE exocytosis

Jenna L. Jewell, Eunjin Oh, Latha Ramalingam, Michael A. Kalwat, Vincent S. Tagliabracci, Lixuan Tackett, Jeffrey S. Elmendorf, Debbie C. Thurmond

Research output: Contribution to journalArticle

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Abstract

How the Sec1/Munc18-syntaxin complex might transition to form the SNARE core complex remains unclear. Toward this, Munc18c tyrosine phosphorylation has been correlated with its dissociation from syntaxin 4. Using 3T3-L1 adipocytes subjected to small interfering ribonucleic acid reduction of Munc18c as a model of impaired insulin-stimulated GLUT4 vesicle exocytosis, we found that coordinate expression of Munc18c-wild type or select phosphomimetic Munc18c mutants, but not phosphodefective mutants, restored GLUT4 vesicle exocytosis, suggesting a requirement for Munc18c tyrosine phosphorylation at Tyr219 and Tyr521. Surprisingly, the insulin receptor (IR) tyrosine kinase was found to target Munc18c at Tyr521 in vitro, rapidly binding and phosphorylating endogenous Munc18c within adipocytes and skeletal muscle. IR, but not phosphatidylinositol 3-kinase, activation was required. Altogether, we identify IR as the first known tyrosine kinase for Munc18c as part of a new insulin-signaling step in GLUT4 vesicle exocytosis, exemplifying a new model for the coordination of SNARE assembly and vesicle mobilization events in response to a single extracellular stimulus.

Original languageEnglish (US)
Pages (from-to)185-199
Number of pages15
JournalJournal of Cell Biology
Volume193
Issue number1
DOIs
StatePublished - Apr 4 2011

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SNARE Proteins
Insulin Receptor
Exocytosis
Qa-SNARE Proteins
Phosphorylation
Adipocytes
Tyrosine
Phosphatidylinositol 3-Kinase
Insulin
Protein-Tyrosine Kinases
Skeletal Muscle
RNA

ASJC Scopus subject areas

  • Cell Biology

Cite this

Jewell, J. L., Oh, E., Ramalingam, L., Kalwat, M. A., Tagliabracci, V. S., Tackett, L., ... Thurmond, D. C. (2011). Munc18c phosphorylation by the insulin receptor links cell signaling directly to SNARE exocytosis. Journal of Cell Biology, 193(1), 185-199. https://doi.org/10.1083/jcb.201007176

Munc18c phosphorylation by the insulin receptor links cell signaling directly to SNARE exocytosis. / Jewell, Jenna L.; Oh, Eunjin; Ramalingam, Latha; Kalwat, Michael A.; Tagliabracci, Vincent S.; Tackett, Lixuan; Elmendorf, Jeffrey S.; Thurmond, Debbie C.

In: Journal of Cell Biology, Vol. 193, No. 1, 04.04.2011, p. 185-199.

Research output: Contribution to journalArticle

Jewell, JL, Oh, E, Ramalingam, L, Kalwat, MA, Tagliabracci, VS, Tackett, L, Elmendorf, JS & Thurmond, DC 2011, 'Munc18c phosphorylation by the insulin receptor links cell signaling directly to SNARE exocytosis', Journal of Cell Biology, vol. 193, no. 1, pp. 185-199. https://doi.org/10.1083/jcb.201007176
Jewell JL, Oh E, Ramalingam L, Kalwat MA, Tagliabracci VS, Tackett L et al. Munc18c phosphorylation by the insulin receptor links cell signaling directly to SNARE exocytosis. Journal of Cell Biology. 2011 Apr 4;193(1):185-199. https://doi.org/10.1083/jcb.201007176
Jewell, Jenna L. ; Oh, Eunjin ; Ramalingam, Latha ; Kalwat, Michael A. ; Tagliabracci, Vincent S. ; Tackett, Lixuan ; Elmendorf, Jeffrey S. ; Thurmond, Debbie C. / Munc18c phosphorylation by the insulin receptor links cell signaling directly to SNARE exocytosis. In: Journal of Cell Biology. 2011 ; Vol. 193, No. 1. pp. 185-199.
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