Murine amyloid protein AA in casein induced experimental amyloidosis

M. Skinner, T. Shirahama, Merrill Benson, A. S. Cohen

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Amyloidosis was induced in mice by 25 subcutaneous injections of casein. The splenic amyloid fibrils were identified by electron microscopy to be closely associated with reticular cells. After isolation of the fibrils by simple physical techniques, their ultrastructure revealed single filaments of 80 to 100 Å width, which were rigid, nonbranching, and of indeterminate length. This is comparable to previous studies on human preparations. The amyloid fibrils were dissociated by solution in guanidine and chromatography. The resultant amyloid fibril protein was characterized as to its molecular weight, amino acid analysis, and aminoterminal sequence. It was thus definitely identified as protein AA, the major component of secondary amyloidosis. An antibody to this protein, murine AA, identified a cross-reacting mouse serum protein SAA and indicated a species specificity when tested against human preparations. A comparison is made with the AA protein in another murine model as well as AA proteins from human, guinea pig, monkey, and mink amyloidosis.

Original languageEnglish (US)
Pages (from-to)420-427
Number of pages8
JournalLaboratory Investigation
Volume36
Issue number4
StatePublished - 1977
Externally publishedYes

Fingerprint

Serum Amyloid A Protein
Amyloidosis
Caseins
Amyloid
Proteins
Amyloidogenic Proteins
Species Specificity
Mink
Guanidine
Protein Sequence Analysis
Subcutaneous Injections
Haplorhini
Chromatography
Blood Proteins
Electron Microscopy
Guinea Pigs
Molecular Weight
Antibodies

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Skinner, M., Shirahama, T., Benson, M., & Cohen, A. S. (1977). Murine amyloid protein AA in casein induced experimental amyloidosis. Laboratory Investigation, 36(4), 420-427.

Murine amyloid protein AA in casein induced experimental amyloidosis. / Skinner, M.; Shirahama, T.; Benson, Merrill; Cohen, A. S.

In: Laboratory Investigation, Vol. 36, No. 4, 1977, p. 420-427.

Research output: Contribution to journalArticle

Skinner, M, Shirahama, T, Benson, M & Cohen, AS 1977, 'Murine amyloid protein AA in casein induced experimental amyloidosis', Laboratory Investigation, vol. 36, no. 4, pp. 420-427.
Skinner, M. ; Shirahama, T. ; Benson, Merrill ; Cohen, A. S. / Murine amyloid protein AA in casein induced experimental amyloidosis. In: Laboratory Investigation. 1977 ; Vol. 36, No. 4. pp. 420-427.
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