Muscarinic receptor subtypes in canine trachea

J. F. Brichant, D. O. Warner, Susan Gunst, K. Rehder

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Prejunctional and postjunctional muscarinic receptor subtypes were characterized in canine trachealis muscle strips. In vitro contractile responses of muscle strips to acetylcholine or electric field stimulation were determined in the absence and the presence of gallamine, pirenzepine, and 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP). Gallamine had no effect on the contractile response to acetylcholine but enhanced the contractile response to electric field stimulation. Pirenzepine and 4-DAMP reduced the contractile response to acetylcholine and electric field stimulation. The pA2 value for pirenzepine vs. acetylcholine [7.18 ± 0.59 (SD)] was consistent with the affinity of pirenzepine for M2 or M3-receptors; whereas the pA2 value for 4-DAMP vs. acetylcholine (8.92 ± 0.42) and the extremely low affinity of gallamine indicated postjunctional muscarinic receptors of the M3 subtype. The enhancement of the contractile response to electric field stimulation by gallamine suggested the presence of M2-prejunctional receptors.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume258
Issue number6 2-3
StatePublished - 1990
Externally publishedYes

Fingerprint

Gallamine Triethiodide
Muscarinic Receptors
Pirenzepine
Trachea
Acetylcholine
Canidae
Electric Stimulation
Muscarinic M3 Receptors
Muscles
4-diphenylacetoxy-1,1-dimethylpiperidinium

Keywords

  • 4-diphenylacetoxy-N-methylpiperidine
  • airways
  • gallamine
  • methiodide
  • muscarinic antagonists
  • pirenzepine

ASJC Scopus subject areas

  • Cell Biology
  • Physiology
  • Pulmonary and Respiratory Medicine

Cite this

Muscarinic receptor subtypes in canine trachea. / Brichant, J. F.; Warner, D. O.; Gunst, Susan; Rehder, K.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 258, No. 6 2-3, 1990.

Research output: Contribution to journalArticle

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