Muscle and tendon size relationships in a paralyzed chick embryo model of clubfoot

John A. Germiller, Amy L. Lerner, Rodney J. Pacifico, Randall Loder, Robert N. Hensinger

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Clubfoot is a birth defect that may be related to muscle weakness or imbalance. The purpose of this study was to examine the relationships among muscle and tendon size and embryonic motility in a paralyzed chick embryo model of clubfoot and arthrogryposis. Decamethonium bromide, a neuromuscular blocking agent, was administered to a series of embryos in five dosage groups, producing a cohort of embryos with various degrees of paralysis and atrophy of tendons and muscle. Embryonic movement frequency was monitored, and after death in late gestation, the cross-sectional areas of the calf musculature and the gastrocnemius tendon proximal to the ankle were measured histologically. Significant correlations were found between embryonic motility and both muscle (r2 = 0.52) and tendon (r2 = 0.77) areas. In addition, a significant correlation (r2 = 0.74) was found between muscle and tendon areas, suggesting that a measurement of one may b used to predict the other.

Original languageEnglish (US)
Pages (from-to)314-318
Number of pages5
JournalJournal of Pediatric Orthopaedics
Volume18
Issue number3
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

Clubfoot
Chick Embryo
Tendons
Muscles
Embryonic Structures
Arthrogryposis
Neuromuscular Blocking Agents
Muscular Atrophy
Muscle Weakness
Ankle
Paralysis
Pregnancy

Keywords

  • Atrophy
  • Clubfoot
  • Muscle
  • Paralysis
  • Tendon

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Surgery
  • Orthopedics and Sports Medicine

Cite this

Muscle and tendon size relationships in a paralyzed chick embryo model of clubfoot. / Germiller, John A.; Lerner, Amy L.; Pacifico, Rodney J.; Loder, Randall; Hensinger, Robert N.

In: Journal of Pediatric Orthopaedics, Vol. 18, No. 3, 1998, p. 314-318.

Research output: Contribution to journalArticle

Germiller, John A. ; Lerner, Amy L. ; Pacifico, Rodney J. ; Loder, Randall ; Hensinger, Robert N. / Muscle and tendon size relationships in a paralyzed chick embryo model of clubfoot. In: Journal of Pediatric Orthopaedics. 1998 ; Vol. 18, No. 3. pp. 314-318.
@article{29a950c8ecf244829ffdd740f0679faa,
title = "Muscle and tendon size relationships in a paralyzed chick embryo model of clubfoot",
abstract = "Clubfoot is a birth defect that may be related to muscle weakness or imbalance. The purpose of this study was to examine the relationships among muscle and tendon size and embryonic motility in a paralyzed chick embryo model of clubfoot and arthrogryposis. Decamethonium bromide, a neuromuscular blocking agent, was administered to a series of embryos in five dosage groups, producing a cohort of embryos with various degrees of paralysis and atrophy of tendons and muscle. Embryonic movement frequency was monitored, and after death in late gestation, the cross-sectional areas of the calf musculature and the gastrocnemius tendon proximal to the ankle were measured histologically. Significant correlations were found between embryonic motility and both muscle (r2 = 0.52) and tendon (r2 = 0.77) areas. In addition, a significant correlation (r2 = 0.74) was found between muscle and tendon areas, suggesting that a measurement of one may b used to predict the other.",
keywords = "Atrophy, Clubfoot, Muscle, Paralysis, Tendon",
author = "Germiller, {John A.} and Lerner, {Amy L.} and Pacifico, {Rodney J.} and Randall Loder and Hensinger, {Robert N.}",
year = "1998",
doi = "10.1097/00004694-199805000-00008",
language = "English (US)",
volume = "18",
pages = "314--318",
journal = "Journal of Pediatric Orthopaedics",
issn = "0271-6798",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Muscle and tendon size relationships in a paralyzed chick embryo model of clubfoot

AU - Germiller, John A.

AU - Lerner, Amy L.

AU - Pacifico, Rodney J.

AU - Loder, Randall

AU - Hensinger, Robert N.

PY - 1998

Y1 - 1998

N2 - Clubfoot is a birth defect that may be related to muscle weakness or imbalance. The purpose of this study was to examine the relationships among muscle and tendon size and embryonic motility in a paralyzed chick embryo model of clubfoot and arthrogryposis. Decamethonium bromide, a neuromuscular blocking agent, was administered to a series of embryos in five dosage groups, producing a cohort of embryos with various degrees of paralysis and atrophy of tendons and muscle. Embryonic movement frequency was monitored, and after death in late gestation, the cross-sectional areas of the calf musculature and the gastrocnemius tendon proximal to the ankle were measured histologically. Significant correlations were found between embryonic motility and both muscle (r2 = 0.52) and tendon (r2 = 0.77) areas. In addition, a significant correlation (r2 = 0.74) was found between muscle and tendon areas, suggesting that a measurement of one may b used to predict the other.

AB - Clubfoot is a birth defect that may be related to muscle weakness or imbalance. The purpose of this study was to examine the relationships among muscle and tendon size and embryonic motility in a paralyzed chick embryo model of clubfoot and arthrogryposis. Decamethonium bromide, a neuromuscular blocking agent, was administered to a series of embryos in five dosage groups, producing a cohort of embryos with various degrees of paralysis and atrophy of tendons and muscle. Embryonic movement frequency was monitored, and after death in late gestation, the cross-sectional areas of the calf musculature and the gastrocnemius tendon proximal to the ankle were measured histologically. Significant correlations were found between embryonic motility and both muscle (r2 = 0.52) and tendon (r2 = 0.77) areas. In addition, a significant correlation (r2 = 0.74) was found between muscle and tendon areas, suggesting that a measurement of one may b used to predict the other.

KW - Atrophy

KW - Clubfoot

KW - Muscle

KW - Paralysis

KW - Tendon

UR - http://www.scopus.com/inward/record.url?scp=0031948307&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031948307&partnerID=8YFLogxK

U2 - 10.1097/00004694-199805000-00008

DO - 10.1097/00004694-199805000-00008

M3 - Article

C2 - 9600555

AN - SCOPUS:0031948307

VL - 18

SP - 314

EP - 318

JO - Journal of Pediatric Orthopaedics

JF - Journal of Pediatric Orthopaedics

SN - 0271-6798

IS - 3

ER -