Muscle-derived CD45-Sca-1+c-Kit- progenitor cells give rise to skeletal muscle myotubes in vitro

Cassandre L. Royer, Jonathan C. Howell, Paul R. Morrison, Edward Srour, Mervin Yoder

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A stem cell population isolated from murine skeletal muscle has recently been shown to differentiate into hematopoietic cells after transplantation in vivo. In the present study, we tested the hypothesis that this cell population would also, under appropriate culture conditions, differentiate into skeletal muscle cells in vitro. Lower-extremity skeletal muscle tissue isolated from 3- to 4-wk-old mice was dissected free from bone and vessels, enzymatically digested, and flow cytometrically sorted to yield CD45-Sca-1+c-Kit- (S+) cells. These cells were further sorted into CD34+ and CD34- fractions and examined for skeletal, cardiac, and hematopoietic lineage-specific messenger RNA (mRNA) transcripts immediately after isolation and after a 10- to 14-d culture period. Freshly isolated S+CD34+ cells lacked expression of skeletal-, cardiac-, or hematopoietic-specific mRNA transcripts, whereas S+CD34- cells expressed c-met, a marker for skeletal muscle satellite cells. During 10-14 d in culture, both S+CD34+ and S+CD34- cell populations underwent a period of attachment followed by elongation and, ultimately, fusion to create large multinucleated contractile myotubes expressing skeletal muscle lineage mRNA transcripts but not hematopoietic or cardiac lineage transcripts. We conclude that murine skeletal muscle possesses two populations of progenitor cells that can be directly isolated. One population expressing the phenotype S+CD34- may contain satellite cells, whereas the S+CD34+ population is devoid of satellite cell markers. Both populations possess the ability to differentiate into skeletal muscle cells in vitro.

Original languageEnglish
Pages (from-to)512-517
Number of pages6
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume38
Issue number9
StatePublished - Oct 2002

Fingerprint

Skeletal Muscle Fibers
Muscle
Skeletal Muscle
Stem Cells
Muscles
Cells
Population
Satellites
Cell culture
Messenger RNA
Muscle Cells
Skeletal Muscle Satellite Cells
In Vitro Techniques
Stem cells
Cell Transplantation
Elongation
Bone
Fusion reactions
Lower Extremity
Tissue

Keywords

  • Gene expression
  • Satellite cell
  • Skeletal muscle
  • Stem cell

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Muscle-derived CD45-Sca-1+c-Kit- progenitor cells give rise to skeletal muscle myotubes in vitro. / Royer, Cassandre L.; Howell, Jonathan C.; Morrison, Paul R.; Srour, Edward; Yoder, Mervin.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 38, No. 9, 10.2002, p. 512-517.

Research output: Contribution to journalArticle

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