Muscle-derived Gr1dimCD11b+ cells enhance neovascularization in an ischemic hind limb mouse model

Jeong A. Kim, Keith March, Hee Don Chae, Brian Johnstone, Su Jung Park, Todd Cook, Stephanie Merfeld-Clauss, Hal Broxmeyer

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Gr1+CD11b+ cells are characterized as myeloid-derived suppressor cells potentially involved in angiogenesis. We demonstrate that Gr1+CD11b+ cells isolated from ischemic muscle in a hind-limb ischemic C57BL/6 mouse model play a role in vessel formation after ischemic injury. Gr1dimCD11b+ cells, a subpopulation of Gr1+CD11b+ cells, within skeletal muscle were increased in context of ischemia. Strikingly, astrocyte-plexus formed from muscle-derived Gr1dimCD11b+ cells in Matrigel culture, followed by formation of isolectin and von Willebrand Factor - expressing cells, similar to that reported for angiogenesis in retina. When isolated muscle-derived Gr1 dimCD11b+ cells were injected into ischemic muscles, recovery of blood flow was significantly enhanced and these cells were incorporated into vessel walls. This suggests that Gr1dimCD11b + cells are recruited into ischemic regions after ischemia and may be involved in angiogenesis by their capacity to generate vascular cells.

Original languageEnglish
Pages (from-to)1623-1626
Number of pages4
JournalBlood
Volume116
Issue number9
DOIs
StatePublished - Sep 2 2010

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Muscle
Extremities
Muscles
von Willebrand Factor
Lectins
Blood
Ischemia
Recovery
Inbred C57BL Mouse
Astrocytes
Blood Vessels
Retina
Skeletal Muscle
Wounds and Injuries

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology
  • Medicine(all)

Cite this

Muscle-derived Gr1dimCD11b+ cells enhance neovascularization in an ischemic hind limb mouse model. / Kim, Jeong A.; March, Keith; Chae, Hee Don; Johnstone, Brian; Park, Su Jung; Cook, Todd; Merfeld-Clauss, Stephanie; Broxmeyer, Hal.

In: Blood, Vol. 116, No. 9, 02.09.2010, p. 1623-1626.

Research output: Contribution to journalArticle

Kim, JA, March, K, Chae, HD, Johnstone, B, Park, SJ, Cook, T, Merfeld-Clauss, S & Broxmeyer, H 2010, 'Muscle-derived Gr1dimCD11b+ cells enhance neovascularization in an ischemic hind limb mouse model', Blood, vol. 116, no. 9, pp. 1623-1626. https://doi.org/10.1182/blood-2009-08-237040
Kim, Jeong A. ; March, Keith ; Chae, Hee Don ; Johnstone, Brian ; Park, Su Jung ; Cook, Todd ; Merfeld-Clauss, Stephanie ; Broxmeyer, Hal. / Muscle-derived Gr1dimCD11b+ cells enhance neovascularization in an ischemic hind limb mouse model. In: Blood. 2010 ; Vol. 116, No. 9. pp. 1623-1626.
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