Muscle-derived Gr1dimCD11b+ cells enhance neovascularization in an ischemic hind limb mouse model

Jeong A. Kim, Keith March, Hee Don Chae, Brian Johnstone, Su Jung Park, Todd Cook, Stephanie Merfeld-Clauss, Hal E. Broxmeyer

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Gr1+CD11b+ cells are characterized as myeloid-derived suppressor cells potentially involved in angiogenesis. We demonstrate that Gr1+CD11b+ cells isolated from ischemic muscle in a hind-limb ischemic C57BL/6 mouse model play a role in vessel formation after ischemic injury. Gr1dimCD11b+ cells, a subpopulation of Gr1+CD11b+ cells, within skeletal muscle were increased in context of ischemia. Strikingly, astrocyte-plexus formed from muscle-derived Gr1dimCD11b+ cells in Matrigel culture, followed by formation of isolectin and von Willebrand Factor - expressing cells, similar to that reported for angiogenesis in retina. When isolated muscle-derived Gr1 dimCD11b+ cells were injected into ischemic muscles, recovery of blood flow was significantly enhanced and these cells were incorporated into vessel walls. This suggests that Gr1dimCD11b + cells are recruited into ischemic regions after ischemia and may be involved in angiogenesis by their capacity to generate vascular cells.

Original languageEnglish (US)
Pages (from-to)1623-1626
Number of pages4
JournalBlood
Volume116
Issue number9
DOIs
StatePublished - Sep 2 2010

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ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Kim, J. A., March, K., Chae, H. D., Johnstone, B., Park, S. J., Cook, T., Merfeld-Clauss, S., & Broxmeyer, H. E. (2010). Muscle-derived Gr1dimCD11b+ cells enhance neovascularization in an ischemic hind limb mouse model. Blood, 116(9), 1623-1626. https://doi.org/10.1182/blood-2009-08-237040