Mushroom Ganoderma lucidum Prevents Colitis-Associated Carcinogenesis in Mice

Daniel Sliva, Jagadish Loganathan, Jiahua Jiang, Andrej Jedinak, John G. Lamb, Colin Terry, Lee Ann Baldridge, Jiri Adamec, George Sandusky, Shailesh Dudhgaonkar

Research output: Contribution to journalArticle

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Abstract

Background: Epidemiological studies suggest that mushroom intake is inversely correlated with gastric, gastrointestinal and breast cancers. We have recently demonstrated anticancer and anti-inflammatory activity of triterpene extract isolated from mushroom Ganoderma lucidum (GLT). The aim of the present study was to evaluate whether GLT prevents colitis-associated carcinogenesis in mice. Methods/Principal Findings: Colon carcinogenesis was induced by the food-borne carcinogen (2-Amino-1-methyl-6-phenylimidazol[4,5-b]pyridine [PhIP]) and inflammation (dextran sodium sulfate [DSS]) in mice. Mice were treated with 0, 100, 300 and 500 mg GLT/kg of body weight 3 times per week for 4 months. Cell proliferation, expression of cyclin D1 and COX-2 and macrophage infiltration was assessed by immunohistochemistry. The effect of GLT on XRE/AhR, PXR and rPXR was evaluated by the reporter gene assays. Expression of metabolizing enzymes CYP1A2, CYP3A1 and CYP3A4 in colon tissue was determined by immunohistochemistry. GLT treatment significantly suppressed focal hyperplasia, aberrant crypt foci (ACF) formation and tumor formation in mice exposed to PhIP/DSS. The anti-proliferative effects of GLT were further confirmed by the decreased staining with Ki-67 in colon tissues. PhIP/DSS-induced colon inflammation was demonstrated by the significant shortening of the large intestine and macrophage infiltrations, whereas GLT treatment prevented the shortening of colon lengths, and reduced infiltration of macrophages in colon tissue. GLT treatment also significantly down-regulated PhIP/DSS-dependent expression of cyclin D1, COX-2, CYP1A2 and CYP3A4 in colon tissue. Conclusions: Our data suggest that GLT could be considered as an alternative dietary approach for the prevention of colitis-associated cancer.

Original languageEnglish
Article numbere47873
JournalPLoS One
Volume7
Issue number10
DOIs
StatePublished - Oct 30 2012

Fingerprint

Reishi
Ganoderma lucidum
Dextran Sulfate
Agaricales
colitis
Colitis
mushrooms
carcinogenesis
colon
Macrophages
Colon
Carcinogenesis
Infiltration
Tissue
sodium sulfate
Cytochrome P-450 CYP3A
Cytochrome P-450 CYP1A2
dextran
Cyclin D1
mice

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Sliva, D., Loganathan, J., Jiang, J., Jedinak, A., Lamb, J. G., Terry, C., ... Dudhgaonkar, S. (2012). Mushroom Ganoderma lucidum Prevents Colitis-Associated Carcinogenesis in Mice. PLoS One, 7(10), [e47873]. https://doi.org/10.1371/journal.pone.0047873

Mushroom Ganoderma lucidum Prevents Colitis-Associated Carcinogenesis in Mice. / Sliva, Daniel; Loganathan, Jagadish; Jiang, Jiahua; Jedinak, Andrej; Lamb, John G.; Terry, Colin; Baldridge, Lee Ann; Adamec, Jiri; Sandusky, George; Dudhgaonkar, Shailesh.

In: PLoS One, Vol. 7, No. 10, e47873, 30.10.2012.

Research output: Contribution to journalArticle

Sliva, D, Loganathan, J, Jiang, J, Jedinak, A, Lamb, JG, Terry, C, Baldridge, LA, Adamec, J, Sandusky, G & Dudhgaonkar, S 2012, 'Mushroom Ganoderma lucidum Prevents Colitis-Associated Carcinogenesis in Mice', PLoS One, vol. 7, no. 10, e47873. https://doi.org/10.1371/journal.pone.0047873
Sliva D, Loganathan J, Jiang J, Jedinak A, Lamb JG, Terry C et al. Mushroom Ganoderma lucidum Prevents Colitis-Associated Carcinogenesis in Mice. PLoS One. 2012 Oct 30;7(10). e47873. https://doi.org/10.1371/journal.pone.0047873
Sliva, Daniel ; Loganathan, Jagadish ; Jiang, Jiahua ; Jedinak, Andrej ; Lamb, John G. ; Terry, Colin ; Baldridge, Lee Ann ; Adamec, Jiri ; Sandusky, George ; Dudhgaonkar, Shailesh. / Mushroom Ganoderma lucidum Prevents Colitis-Associated Carcinogenesis in Mice. In: PLoS One. 2012 ; Vol. 7, No. 10.
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