Mutagenesis of the a subunit of the F1F0-ATPase from Escherichia coli. Mutations at Glu-196, Pro-190, and Ser-199

S. B. Vik, B. D. Cain, Kristin Chun, R. D. Simoni

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Abstract

In an attempt to identify amino acid residues involved in proton translocation by the F0 sector of the Escherichia coli F1F0-ATPase, 16 mutations at the carboxyl-terminal third of the a subunit have been isolated, and their phenotypes have been partially characterized. Thirteen mutations were constructed by 'cassette' mutagenesis at two highly conserved residues, a(glu196) and a(pro190). Two mutations were products of oligonucleotide-directed mutagenesis of a portion of the uncB gene cloned into an M13 vector. One mutation was isolated after in vitro mutagenesis of the entire uncB gene in a plasmid vector with hydroxylamine. Amino acid substitutions for aglu 196 (Asp, Gln, His, Asn, Lys, Ala, Ser, Pro) affect ATP-driven proton translocation and passive proton permeability by F0 to varying extents, but do not prevent growth on minimal succinate media. Amino acid substitutions of glutamine or arginine for a(pro190) affect F1F0-ATPase assembly and eliminate ATP-driven proton translocation, while the substitution of asparagine at this position does not significantly affect either assembly or proton translocation. The substitution of amino acids threonine or alanine for a(ser199) causes no detectable phenotypic change from wild type. These and other mutations are discussed in terms of the assembly, structure, and function of the a subunit. It is concluded that a(glu196) and a(pro190) are not obligate components of the proton channel, but that they affect proton translocation indirectly.

Original languageEnglish (US)
Pages (from-to)6599-6605
Number of pages7
JournalJournal of Biological Chemistry
Volume263
Issue number14
StatePublished - 1988
Externally publishedYes

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Mutagenesis
Escherichia coli
Adenosine Triphosphatases
Protons
Mutation
Substitution reactions
Amino Acid Substitution
Amino Acids
Genes
Adenosine Triphosphate
Hydroxylamine
Insertional Mutagenesis
Asparagine
Succinic Acid
Threonine
Site-Directed Mutagenesis
Glutamine
Oligonucleotides
Alanine
Arginine

ASJC Scopus subject areas

  • Biochemistry

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Mutagenesis of the a subunit of the F1F0-ATPase from Escherichia coli. Mutations at Glu-196, Pro-190, and Ser-199. / Vik, S. B.; Cain, B. D.; Chun, Kristin; Simoni, R. D.

In: Journal of Biological Chemistry, Vol. 263, No. 14, 1988, p. 6599-6605.

Research output: Contribution to journalArticle

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