Mutation of the uracil DNA glycosylase gene detected in glioblastoma

Young Wan Moon, Won Sang Park, Alexander O. Vortmeyer, Robert J. Weil, Youn Soo Lee, Thomas A. Winters, Zhengping Zhuang, Brian G. Fuller

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Despite extensive characterization of genetic changes in gliomas, the underlying etiology of these tumors remains largely unknown. Spontaneous DNA damage due to hydrolysis, methylation, and oxidation is a frequent event in the brain. Failure of DNA repair following this damage may contribute to tumorigenesis of gliomas. Uracil DNA glycosylase (UDG), an enzyme which excises uracil from DNA, is an important component of the base excision repair pathway. The sequence of a human homologue of uracil DNA glycosylase gene (UNG) has been recently identified. We performed PCR-based SSCP mutational analysis of UNG in 11 sporadic gliomas (six glioblastomas, two anaplastic astrocytomas, and three oligodendrogliomas) and eight glioblastoma cell lines. One out of six sporadic glioblastomas had a point mutation in exon 3, which resulted in a missense mutation in codon 143. None of the eight glioblastoma cell lines or the five non-glioblastoma sporadic gliomas showed a mutation. Genetic alterations of UNG may play a role in the development of a subset of primary glioblastomas. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)191-196
Number of pages6
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume421
Issue number2
DOIs
StatePublished - Nov 3 1998
Externally publishedYes

Keywords

  • Glioblastoma multiforme
  • Single strand conformation polymorphism analysis
  • Uracil DNA glycosylase gene

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

Fingerprint Dive into the research topics of 'Mutation of the uracil DNA glycosylase gene detected in glioblastoma'. Together they form a unique fingerprint.

  • Cite this