Mutational analysis of bovine papillomavirus E6 gene

K. H. Vousden, E. J. Androphy, J. T. Schiller, D. R. Lowy

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

The bovine papillomavirus E6 gene can independently transform mouse C127 cells. To characterize E6 in greater detail, we created 16 site-directed mutations in E6, including substitution mutations in the cysteine codons of the four Cys-X-X-Cys motifs that are conserved in all papillomavirus E6 proteins. Proteins mutated in six of the seven cysteines tested, as well as those lacking the nonconserved C-terminus, were stable in transfected cells but were unable to induce morphological transformation, indicating that these amino acids play an important role in the function of E6.

Original languageEnglish (US)
Pages (from-to)2340-2342
Number of pages3
JournalJournal of virology
Volume63
Issue number5
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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