Mutational analysis of the PRP4 protein of Saccharomyces cerevisiae suggests domain structure and snRNP interactions

J. Hu, Yan Xu, K. Schappert, T. Harrington, A. Wang, R. Braga, J. Mogridge, J. D. Friesen

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The PRP4 protein of Saccharomyces cerevisiae is an essential part of the U4/U6 snRNP, a component of the mRNA splicing apparatus. As an approach to the determination of structure-function relationships in the PRP4 protein, we have isolated more than fifty new alleles of the PRP4 gene through random and site-directed mutagenesis, and have analyzed the phenotypes of many of them. Twelve of the fourteen single-point mutations that give rise to temperature-sensitive (ts) or null phenotypes are located in the portion of the PRP4 gene that corresponds to the β-transducin-like region of the protein; the remaining two are located in the central portion of the gene, one of them in an arginine - lysine-rich region. Nine additional deletion or deletion/insertion mutations were isolated at both the amino- and carboxy-termini. These data show that the amino-terminal region (108 amino acids) of PRP4 is non-essential, while the carboxy-terminal region is essential up to the penultimate amino acid. A deletion of one entire β-transducin-like repeat (the third of five) resulted in a null phenotype. All ts mutants show a first-step defect in the splicing of U3 snRNA primary transcript in vivo at the non-permissive temperature. The effects on prp4 mutant growth of increased copy-number of mutant prp4 genes themselves, and of genes for other components of the U4/U6 snRNP (PRP3 and U6 snRNA) have also been studied. We suggest that the PRP4 protein has at least three domains: a non-essential amino-terminal segment of at least 108 amino acids, a central basic region of about 140 residues that is relatively refractile to mutation and might be involved in RNA interaction, and an essential carboxy-terminal region of about 210 residues with the five repeat-regions that are similar to β-transducins, which might be involved in protein-protein interaction. A model of interactions of snRNP components suggested by these results is presented.

Original languageEnglish (US)
Pages (from-to)1724-1734
Number of pages11
JournalNucleic Acids Research
Volume22
Issue number9
StatePublished - May 11 1994
Externally publishedYes

Fingerprint

Small Nuclear Ribonucleoproteins
Saccharomyces cerevisiae Proteins
Saccharomyces Cerevisiae
Yeast
Transducin
Genes
U4-U6 Small Nuclear Ribonucleoproteins
Proteins
Protein
Interaction
Gene
Amino acids
Mutant
Phenotype
Deletion
Amino Acids
Temperature
arginyllysine
Mutation
INDEL Mutation

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Hu, J., Xu, Y., Schappert, K., Harrington, T., Wang, A., Braga, R., ... Friesen, J. D. (1994). Mutational analysis of the PRP4 protein of Saccharomyces cerevisiae suggests domain structure and snRNP interactions. Nucleic Acids Research, 22(9), 1724-1734.

Mutational analysis of the PRP4 protein of Saccharomyces cerevisiae suggests domain structure and snRNP interactions. / Hu, J.; Xu, Yan; Schappert, K.; Harrington, T.; Wang, A.; Braga, R.; Mogridge, J.; Friesen, J. D.

In: Nucleic Acids Research, Vol. 22, No. 9, 11.05.1994, p. 1724-1734.

Research output: Contribution to journalArticle

Hu, J, Xu, Y, Schappert, K, Harrington, T, Wang, A, Braga, R, Mogridge, J & Friesen, JD 1994, 'Mutational analysis of the PRP4 protein of Saccharomyces cerevisiae suggests domain structure and snRNP interactions', Nucleic Acids Research, vol. 22, no. 9, pp. 1724-1734.
Hu, J. ; Xu, Yan ; Schappert, K. ; Harrington, T. ; Wang, A. ; Braga, R. ; Mogridge, J. ; Friesen, J. D. / Mutational analysis of the PRP4 protein of Saccharomyces cerevisiae suggests domain structure and snRNP interactions. In: Nucleic Acids Research. 1994 ; Vol. 22, No. 9. pp. 1724-1734.
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abstract = "The PRP4 protein of Saccharomyces cerevisiae is an essential part of the U4/U6 snRNP, a component of the mRNA splicing apparatus. As an approach to the determination of structure-function relationships in the PRP4 protein, we have isolated more than fifty new alleles of the PRP4 gene through random and site-directed mutagenesis, and have analyzed the phenotypes of many of them. Twelve of the fourteen single-point mutations that give rise to temperature-sensitive (ts) or null phenotypes are located in the portion of the PRP4 gene that corresponds to the β-transducin-like region of the protein; the remaining two are located in the central portion of the gene, one of them in an arginine - lysine-rich region. Nine additional deletion or deletion/insertion mutations were isolated at both the amino- and carboxy-termini. These data show that the amino-terminal region (108 amino acids) of PRP4 is non-essential, while the carboxy-terminal region is essential up to the penultimate amino acid. A deletion of one entire β-transducin-like repeat (the third of five) resulted in a null phenotype. All ts mutants show a first-step defect in the splicing of U3 snRNA primary transcript in vivo at the non-permissive temperature. The effects on prp4 mutant growth of increased copy-number of mutant prp4 genes themselves, and of genes for other components of the U4/U6 snRNP (PRP3 and U6 snRNA) have also been studied. We suggest that the PRP4 protein has at least three domains: a non-essential amino-terminal segment of at least 108 amino acids, a central basic region of about 140 residues that is relatively refractile to mutation and might be involved in RNA interaction, and an essential carboxy-terminal region of about 210 residues with the five repeat-regions that are similar to β-transducins, which might be involved in protein-protein interaction. A model of interactions of snRNP components suggested by these results is presented.",
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