Mutations altering the structure of epidermal growth factor-like coding sequences at the Drosophila Notch locus

Mark Kelley, Simon Kidd, Walter A. Deutsch, Michael W. Young

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

The Drosophila neurogenic protein Notch is largely composed of tandemly repeated copies of an epidermal growth factor-like sequence. Notch protein contains 36 EGF-like elements, but no two are identical. In the present study, eight mutations are correlated with single amino acid substitutions in EGF-homologous elements of this protein. Genetic analyses of the mutations and comparisons of DNA from mutant and wild-type flies indicate that differentiation of function exists among the tandemly repeated EGF-like sequences and that the total number of repeats and minor variations from the consensus repeat sequence are important for wild-type Notch protein function. One group of EGF-like repeats may be involved in the formation of functional Notch dimers or multimeric proteins. EGF-like elements in a second region of the Notch protein appear to govern its interaction with the product of Enhancer of split, another neurogenic locus of D. melanogaster.

Original languageEnglish (US)
Pages (from-to)539-548
Number of pages10
JournalCell
Volume51
Issue number4
DOIs
StatePublished - Nov 20 1987
Externally publishedYes

Fingerprint

Epidermal Growth Factor
Notch Receptors
Drosophila
Mutation
Consensus Sequence
Amino Acid Substitution
Diptera
Dimers
Proteins
Substitution reactions
Amino Acids
DNA

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Mutations altering the structure of epidermal growth factor-like coding sequences at the Drosophila Notch locus. / Kelley, Mark; Kidd, Simon; Deutsch, Walter A.; Young, Michael W.

In: Cell, Vol. 51, No. 4, 20.11.1987, p. 539-548.

Research output: Contribution to journalArticle

Kelley, Mark ; Kidd, Simon ; Deutsch, Walter A. ; Young, Michael W. / Mutations altering the structure of epidermal growth factor-like coding sequences at the Drosophila Notch locus. In: Cell. 1987 ; Vol. 51, No. 4. pp. 539-548.
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