Mutations in the arginine-rich protein gene, in lung, breast, and prostate cancers, and in squamous cell carcinoma of the head and neck

Ravi Shridhar, Viji Shridhar, Sylvie Rivard, Jill M. Siegfried, Heline Pietraszkiewicz, John Ensley, Robert Pauley, David Grignon, Wael Sakr, Orlando J. Miller, David I. Smith

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Ariginine-rich protein (ARP) is a highly conserved gene that maps to human chromosomal hand 3p21.1. This gene contains an imperfect trinucleotide repeat which encodes a string of arginines. We previously detected a specific mutation (ATG50 → AGG) within this region of the gene in 10 of 21 sporadic renal cell carcinomas. Here, we report the detection of the same mutation in 5 of 21 squamous cell carcinomas of the head and neck, 1 of 2 small cell lung cancer cell lines, 6 of 18 non-small cell lung carcinomas, 9 of 22 breast tumors, and 5 of 13 prostate tumors. This mutation was seen in several early stage tumors and may thus be an early event in tumorigenesis. We also detected a mutation at codon 53 of this gene in both primary and metastatic tumors from one patient. Other nucleotide changes were observed in a few PCR subclones, but their frequency was the same in both tumor and control samples, suggesting that many of these changes were PCR or subcloning artifacts rather than mutations in the tumor cells themselves.

Original languageEnglish (US)
Pages (from-to)5576-5578
Number of pages3
JournalCancer Research
Volume56
Issue number24
StatePublished - Dec 15 1996
Externally publishedYes

Fingerprint

Arginine
Lung Neoplasms
Prostatic Neoplasms
Breast Neoplasms
Mutation
Neoplasms
Proteins
Genes
Trinucleotide Repeats
Polymerase Chain Reaction
Small Cell Lung Carcinoma
Renal Cell Carcinoma
Non-Small Cell Lung Carcinoma
Codon
Artifacts
Prostate
Carcinogenesis
Nucleotides
Hand
Carcinoma, squamous cell of head and neck

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Shridhar, R., Shridhar, V., Rivard, S., Siegfried, J. M., Pietraszkiewicz, H., Ensley, J., ... Smith, D. I. (1996). Mutations in the arginine-rich protein gene, in lung, breast, and prostate cancers, and in squamous cell carcinoma of the head and neck. Cancer Research, 56(24), 5576-5578.

Mutations in the arginine-rich protein gene, in lung, breast, and prostate cancers, and in squamous cell carcinoma of the head and neck. / Shridhar, Ravi; Shridhar, Viji; Rivard, Sylvie; Siegfried, Jill M.; Pietraszkiewicz, Heline; Ensley, John; Pauley, Robert; Grignon, David; Sakr, Wael; Miller, Orlando J.; Smith, David I.

In: Cancer Research, Vol. 56, No. 24, 15.12.1996, p. 5576-5578.

Research output: Contribution to journalArticle

Shridhar, R, Shridhar, V, Rivard, S, Siegfried, JM, Pietraszkiewicz, H, Ensley, J, Pauley, R, Grignon, D, Sakr, W, Miller, OJ & Smith, DI 1996, 'Mutations in the arginine-rich protein gene, in lung, breast, and prostate cancers, and in squamous cell carcinoma of the head and neck', Cancer Research, vol. 56, no. 24, pp. 5576-5578.
Shridhar R, Shridhar V, Rivard S, Siegfried JM, Pietraszkiewicz H, Ensley J et al. Mutations in the arginine-rich protein gene, in lung, breast, and prostate cancers, and in squamous cell carcinoma of the head and neck. Cancer Research. 1996 Dec 15;56(24):5576-5578.
Shridhar, Ravi ; Shridhar, Viji ; Rivard, Sylvie ; Siegfried, Jill M. ; Pietraszkiewicz, Heline ; Ensley, John ; Pauley, Robert ; Grignon, David ; Sakr, Wael ; Miller, Orlando J. ; Smith, David I. / Mutations in the arginine-rich protein gene, in lung, breast, and prostate cancers, and in squamous cell carcinoma of the head and neck. In: Cancer Research. 1996 ; Vol. 56, No. 24. pp. 5576-5578.
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